Physiological relevance of catecholamines produced by the endothelium

• Grant number: 18/24971-9
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: September 01, 2019
• End date: December 31, 2023
• Field of knowledge: Biological Sciences - Pharmacology - Clinical Pharmacology
• Principal Investigator: Gilberto de Nucci
• Grantee: Felipe Fernandes Jacintho
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The endothelium is an organ able to regulate vascular tone through the release of relaxants agents (nitric oxide and prostacyclin - PGI2) or contractile (endothelin - ET1 and prostanoid - PGH2). Despite the physiological concept that catecholamines from neural origin regulate vascular tone, recent pharmacological studies by the group on tissues isolated from reptiles have shown that the endothelium is also a source of catecholamines. Morphological analyzes located the enzyme tyrosine hydroxylase (essential in catecholamine synthesis) in vascular endothelium of aortas and corpus cavernosum of snakes. The same enzyme was also found in human tissue (which has paraganglioma) again confirming the endothelium as a source of catecholamine. The objective of this work is to investigate the physiological role of catecholamines produced by the endothelium in mammals: mouse, rat, rabbit, dog and monkey. It will be used aorta, mesenteric, iliac and pulmonary arteries of the group of animals mentioned for electric field stimulus-induced contraction (EFS) in the presence and absence of phentolamine, guanethidine, tetrodotoxin (TTX) and A-803467 (resistant sodium channel inhibitor) to TTX). Contraction EFS will also be performed on vascular rings without endothelium in healthy animals and in hypertensive rats. Immunohistochemical analyzes will investigate the localization of tyrosine hydroxylase (TH), dopa decarboxylase (DDC), dopa-beta-hydroxylase (DbH) and phenylethanolamine-n-methyltransferase (PNMT) in the endothelium of these vessels, in addition to norepinephrine (NE) and epinephrine (EPI). Groups of SHR rats will be denervated, vessels, blood and urine will be collected for quantification of NE and EPI. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRITTO-JUNIOR, JOSE; CAMPOS, RAFAEL; PEIXOTO, MATHEUS; LIMA, ANTONIO TIAGO; JACINTHO, FELIPE FERNANDES; MONICA, FABIOLA Z.; MORENO, RONILSON AGNALDO; ANTUNES, EDSON; DE NUCCI, GILBERTO. 6-Nitrodopamine is an endogenous selective dopamine receptor antagonist in Chelonoidis carbonaria aorta. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v. 260, p. 7-pg., 2022-07-07. (19/16805-4, 17/15175-1, 18/24971-9, 21/13593-6)

BRITTO-JUNIOR, JOSE; JACINTHO, FELIPE FERNANDES; CAMPOS, RAFAEL; ARAUJO PINHEIRO, DAVID HALEN; FIGUEIREDO MURARI, GUILHERME M.; DE SOUZA, VALERIA B.; SCHENKA, ANDRE A.; MONICA, FABIOLA Z.; MORENO, RONILSON AGNALDO; ANTUNES, EDSON; et al. The basal release of endothelium-derived catecholamines regulates the contractions of Chelonoidis carbonaria aorta caused by electrical-field stimulation. BIOLOGY OPEN, v. 10, n. 1, JAN 2021. (18/24971-9, 16/04731-8, 17/15175-1)

CAMPOS, RAFAEL; JACINTHO, FELIPE FERNANDES; BRITTO-JUNIOR, JOSE; MONICA, FABIOLA Z.; OLIVEIRA JUSTO, ALBERTO FERNANDO; PUPO, ANDRE SAMPAIO; MORENO, RONILSON AGNALDO; DE SOUZA, VALERIA BARBOSA; SCHENKA, ANDRE ALMEIDA; ANTUNES, EDSON; et al. Endothelium modulates electrical field stimulation-induced contractions of Chelonoidis carbonaria aortic rings. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v. 233, JUL 2020. (18/24971-9, 16/04731-8, 16/09539-8)

BRITTO-JUNIOR, JOSE; RIBEIRO, ANDRE; XIMENES, LUIZ; LIMA, ANTONIO TIAGO; JACINTHO, FELIPE FERNANDES; FREGONESI, ADRIANO; MONICA, FABIOLA Z.; ANTUNES, EDSON; DE NUCCI, GILBERTO. lpha1-adrenergic antagonists block 6-nitrodopamine contractions on the rat isolated epididymal vas deferen. European Journal of Pharmacology, v. 915, JAN 15 2022. (18/24971-9, 17/15175-1, 19/16805-4)

BRITTO-JUNIOR, JOSE; JACINTHO, FELIPE FERNANDES; FIGUEIREDO MURARI, GUILHERME M.; CAMPOS, RAFAEL; MORENO, RONILSON AGNALDO; ANTUNES, EDSON; MONICA, FABIOLA Z.; DE NUCCI, GILBERTO. Electrical field stimulation induces endothelium-dependent contraction of human umbilical cord vessels. Life Sciences, v. 243, FEB 15 2020. (18/24971-9, 17/15175-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

JACINTHO, Felipe Fernandes. Endothelial catecholamines as modulators of vascular reactivity in Chelonoidis carbonaria aorta. 2023. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.