Molecular detection and phylogenetic analysis of zika virus (ZIKV) in non-human primates (PNH) in the State of São Paulo and Paraná, Brazil

• Grant number: 19/05288-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2019
• End date: May 31, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: João Pessoa Araújo Junior
• Grantee: Amanda Haisi
• Host Institution: Instituto de Biotecnologia (IBTEC). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The Zika virus belongs to the genus Flavivirus, family Flaviviridae, and possessing similarity with other arboviruses of interest for the one health. Its discovery occurred in 1947 with the isolation in Rhesus monkey, sentinel in the control of yellow fever in the forest of Uganda. The following year, Aedes africanus were positive in the same region, and from then on, several isolations were carried out on wild mosquitoes in Africa. The main form of transmission of ZIKV occurs by infected mosquito vectors, through the wild cycle involving non-human primates (PNH) and arboreal mosquitoes, and an urban cycle involving humans and anthropophilic mosquitoes of the genus Aedes spp. Recent studies indicate that neotropical PNH may be acting as hosts for maintenance and/or amplification of the virus, however more studies should be performed with these mammals and populations of mosquito vectors to better characterize the circulating strains. Therefore, this work aims at the molecular detection of ZIKV and phylogenetic analysis of samples of whole blood and tissues of neotropical PNH from São Paulo and Paraná states. Samples will be submitted to RT-qPCR technique using a primer specific for the viral envelope (ZIKV 1086). For massive sequencing, libraries will be prepared with Illumina kits from 24 RT-qPCR positive samples. The results will be analyzed with the bioinformatics tools. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AMANDA HAISI; STACY WU; NATHALIA ZINI; MARIA LUANA CRISTINY RODRIGUES DA SILVA; CAMILA DANTAS MALOSSI; ZALMIR SILVINO CUBAS; PATRÍCIA HOERNER CUBAS; RODRIGO HIDALGO FRICIELLO TEIXEIRA; MÔNICA SHINNEIDER DE SOUSA; RICARDO BARBOSA LUCENA; et al. Lack of serological and molecular evidences of Zika virus circulation in non-human primates in three states from Brazil. Memórias do Instituto Oswaldo Cruz, v. 117, 2022-09-05. (19/05288-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

HAISI, Amanda. Laboratorial investigation of Zika virus in non-human primates from Paraná, São Paulo and Paraíba states, Brazil. 2021. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.