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Characterization of nonionic cubosomes in the presence of model proteins: a structural approach

Grant number: 19/19426-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2019
Effective date (End): November 30, 2020
Field of knowledge:Physical Sciences and Mathematics - Physics
Principal Investigator:Leandro Ramos Souza Barbosa
Grantee:Amanda Santos Palma
Home Institution: Instituto de Física (IF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

One area of research that has gained much attention in recent years is nanomedicine, with particular attention to drug delivery systems. Among the various nanoparticles used for this purpose, we highlight the systems formed by lipids and polymers, such as liposomes and cubosomes. In this project we will study the structural influence of a model preotein: lysozyme in cubosomes.Lysozyme is an animal-produced antimicrobial enzyme that is part of the immune system. Lysozyme is a glycosidic hydrolase that catalyzes the hydrolysis of 1,4-beta bonds between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan, which is the major component of the gram-positive bacterial cell wall. This hydrolysis, in turn, compromises the integrity of the bacterial cell walls, causing the lysis (and consequently the death) of the bacteria. The main objective of this research project is to build nanostructured systems capable of acting as antimicrobial systems. These systems will be composed of cubosomes in the absence and presence of the enzyme and will be analyzed using several biophysical techniques such as: small angle X-ray scattering (SAXS) and dynamic light scattering (DLS), potential zeta besides essays in vivo.