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Effect of the association of fluoridated toothpaste and mouth rinses with sodium trimetaphosphate on dental demineralization: an in vitro study

Grant number: 19/16300-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2020
Effective date (End): December 31, 2020
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Alberto Carlos Botazzo Delbem
Grantee:Amanda Scarpin Gruba
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


The decline of dental caries in the last two decades has been attributed to the widespread use of fluoride. Two forms of presentation are fluoridated mouthrinses and toothpastes, widely used by the population. The aim of the present study will be to evaluate in vitro the ability of the association between fluoride toothpastes containing 1100 ppm F with mouthrinses supplemented or not with sodium trimetaphosphate (TMP) in reducing tooth enamel demineralization. Bovine blocks (n = 60) will be selected by means of the initial surface hardness (SHi) and then divided into 5 experimental groups (n = 12): 1) Placebo toothpaste (without F / TMP); 2) toothpaste 1100 ppm F, 3) toothpaste 1100 ppm associated with a mouthrinses 100 ppm F, 4) toothpaste 1100 ppm associated with a mouthrinses 225 ppm F and 5) toothpaste 1100 ppm F associated with a mouthrinses 100 ppm F supplemented with 0.4% TMP. The blocks will be treated twice daily with toothpastes and mouthrinses, being submitted to 5 pH cycling for 7 days. After pH cycling, the final surface hardness (SHf), the percentage of surface hardness change (% SH), shall be determined. The data will be submitted to normality and homogeneity tests to use the statistical test most appropriate to the data distribution. Sigmaplot © software statistical software for Windows version 12.0 will be used, with significance at the 5% level.