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Evaluation of Cu / Zn-Superoxide Dismutase (SOD) on antimicrobial photodynamic action

Grant number: 19/26524-2
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2020
Effective date (End): January 31, 2022
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Carla Raquel Fontana Mendonça
Grantee:Vinícius Medeiros Momesso
Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Photodynamic Therapy (PDT) has been shown to be very effective for cancer treatment and antimicrobial therapy, as well as being a non-invasive treatment. In the last decades, studies have been conducted using PDT as monotherapy and also as combined therapy, aiming for optimization of bacterial reduction.It is known that the resistance of microorganisms against antibiotics has increased and the number of deaths per year is expected to increase by 10 million by 2050 due to this fact. Combining PDT with other compounds may result in a decrease in the use of these antimicrobials and in the concentration of photosensitizer used and further increase the effectiveness of PDT. The treatment has as a mechanism the production of oxygen free radicals, one of them being the superoxide radical (O2-), through the concomitant application of a photosensitizer (PS) and a certain light wavelength. In this context, the present study aims at enhancing PDT with the use of Superoxide Dismutase inhibitor (Diethyl dithiocarbamate (DDC)), more specifically Cu / Zn-SOD inhibitor, which acts in the body's defense against stress oxidative via superoxide radical (O2-). Cu / Zn-SOD producing microorganisms as a defense against DNA damage and cell integrity may be susceptible to the combination of treatment proposed in this study. Therefore, the efficacy of PDT with different concentrations of the photosensitizers and the DDC inhibitor will be evaluated, using Escherichia coli ATCC 25922 as a model, which will be tested in both suspension and biofilm. The results will be evaluated by the ANOVA statistical method and others when deemed necessary.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SURUR, AMANDA KOBERSTAIN; MOMESSO, VINICIUS MEDEIROS; LOPES, PEDRO MONTEIRO; FERRISSE, TULIC MORANDIN; FONTANA, CARLA RAQUEL. Assessment of synergism between enzyme inhibition of Cu/Zn-SOD and antimicrobial photodynamic therapy in suspension and E. coli biofilm. Photodiagnosis and Photodynamic Therapy, v. 41, p. 8-pg., . (21/01191-0, 19/26524-2, 13/07276-1)

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