Effect of dietary on the gut microbiota of cecum content of monogastric animals

• Grant number: 20/00239-7
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: March 18, 2020
• End date: July 17, 2020
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Aline Silva Mello Cesar
• Grantee: Julia Dezen Gomes
• Supervisor: Mark Wesley Schilling
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Institution abroad: Mississippi State University (MSU), United States
• Associated to the scholarship: 18/26816-0 - Effect of dietary oleic acid on fatty acid composition and inflammatory response profile in different tissues of pigs, BP.IC

Abstract

The gastrointestinal tract of monogastric animals is predominantly composed of bacteria, forming a complex and dynamic system. The intestinal microbiota acts in symbiosis with the host, regulating absorptive efficiency, length of stay of the bolus in the tract, utilization of some nutrients poorly digestible by the animal's endogenous enzymes, intestinal maturation, antagonism against pathogens, enhancement of intestinal integrity, immunity modulation. and detoxification of certain compounds. Thus arises the need for greater knowledge of the microbiota inhabiting the tract. The complexity of the study of the microbial population is related to the fact that there are an unknown number of bacterial species in the gastrointestinal tract of birds, and the vast majority of those colonizing the caecum are not cultivable. DNA contains all the information necessary to identify a bacterium, and allows us to understand how the mechanisms of adaptation and response of bacterial communities to adverse situations work. Thus, our objectives are to obtain the cecum content of monogastric animals, to evaluate the impact of the generic strain and feeding regime on the gut content of the cecal microbiota. As well as extracting microbial community DNA from cecum specimens, constructing libraries, sequencing them, and using bioinformatics and statistical approach tools to analyze the results. (AU)