Connective tissue reaction induced by BIO-C Pulpo and MTA Repair HP implanted in the rat subcutaneous

• Grant number: 19/17739-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2020
• End date: January 31, 2021
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Paulo Sergio Cerri
• Grantee: José Leandro de Abreu Jampani
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Repair sealers must have ideal physicochemical and biological properties as well as the potential for osteoinduction. MTA is considered the most effective material in tissue repair due to good sealing ability, biocompatibility, and bioactivity. New MTA-containing materials have been launched on the market in an attempt to improve the physicochemical properties of the White MTA (W-MTA). Thus, it has been reported that MTA Repair HP (MTA-HP) has physicochemical properties superior to the W-MTA. BIO-C Pulpo (BIO-C) is new material produced by Angelus that is not yet in the trade. The purpose of this study will be to evaluate the bioactivity and biocompatibility of BIO-C and MTA-HP sealers in comparison with the W-MTA implanted in the rat subcutaneous. Polyethylene tubes filled with BIO-C (n=20), MTA-HP (n=20) and W-MTA (n=20) will be implanted into subcutaneous rats. In the control group GC (n=20), empty tubes will be implanted. After 7, 15, 30 and 60 days, the rats will be sacrificed with anesthetic overdose and the implanted tubes surrounded by connective tissue will be also removed and will be fixed. After fixation, the specimens will be dehydrated, treated with xylene and embedded in paraffin. To evaluate the tissue reaction promoted by materials implanted into subcutaneous, from HE-stained sections the number of inflammatory cells and fibroblasts, and the thickness of the capsules will be obtained. The birefringent collagen content will be quantified from sections subjected to the picrosirius-red and analyzed under a light microscope with polarized filters. Interleukin-6 (IL-6), a pro-inflammatory cytokine, will be detected by immunohistochemical reaction and the number of immunolabelled cells will be computed. The bioactive potential will be evaluated by von Kossa reaction and analysis of unstained sections analyzed under polarized light, which will be performed to detect calcium deposits and calcite crystals, respectively. Some sections, after von Kossa reaction, will be subjected to immunohistochemistry for the detection of alkaline phosphatase, an enzyme produced by mineralized tissue cells. Data will be analyzed by two-way ANOVA and Tukey post-test (pd0.05).

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DELFINO, MATEUS MACHADO; DE ABREU JAMPANI, JOSE LEANDRO; LOPES, CAMILA SOARES; GUERREIRO-TANOMARU, JULIANE M.; TANOMARU-FILHO, MARIO; SASSO-CERRI, ESTELA; CERRI, PAULO S.. Comparison of Bio-C Pulpo and MTA Repair HP with White MTA: effect on liver parameters and evaluation of biocompatibility and bioactivity in rats. International Endodontic Journal, v. 54, n. 9, JUL 2021. (19/17739-5, 18/16848-2)

DELFINO, MATEUS MACHADO; JAMPANI, JOSE LEANDRO DE ABREU; LOPES, CAMILA SOARES; GUERREIRO-TANOMARU, JULIANE MARIA; TANOMARU-FILHO, MARIO; SASSO-CERRI, ESTELA; CERRI, PAULO SERGIO. Participation of fibroblast growth factor-1 and interleukin-10 in connective tissue repair following subcutaneous implantation of bioceramic materials in rats. International Endodontic Journal, v. N/A, p. 17-pg., 2022-11-22. (18/16848-2, 19/17739-5)

QUEIROZ, MARCELA BORSATTO; HANDA INADA, RAFAELA NANAMI; LOPES, CAMILA SOARES; GUERREIRO-TANOMARU, JULIANE MARIA; SASSO-CERRI, ESTELA; TANOMARU-FILHO, MARIO; CERRI, PAULO SERGIO. Bioactive potential of Bio-C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 110, n. 10, p. 12-pg., 2022-05-18. (19/17739-5)

QUEIROZ, MARCELA BORSATTO; RAFAELA INADA, N. H.; DE ABREU JAMPANI, JOSE LEANDRO; GUERREIRO-TANOMARU, JULIANE MARIA; SASSO-CERRI, ESTELA; TANOMARU-FILHO, MARIO; CERRI, PAULO SERGIO. Biocompatibility and bioactive potential of an experimental tricalcium silicate-based cement in comparison with Bio-C repair and MTA Repair HP materials. International Endodontic Journal, v. 56, n. 2, p. 19-pg., 2022-11-13. (19/17739-5)