Abstract
Repair sealers must have ideal physicochemical and biological properties as well as the potential for osteoinduction. MTA is considered the most effective material in tissue repair due to good sealing ability, biocompatibility, and bioactivity. New MTA-containing materials have been launched on the market in an attempt to improve the physicochemical properties of the White MTA (W-MTA). Thus, it has been reported that MTA Repair HP (MTA-HP) has physicochemical properties superior to the W-MTA. BIO-C Pulpo (BIO-C) is new material produced by Angelus that is not yet in the trade. The purpose of this study will be to evaluate the bioactivity and biocompatibility of BIO-C and MTA-HP sealers in comparison with the W-MTA implanted in the rat subcutaneous. Polyethylene tubes filled with BIO-C (n=20), MTA-HP (n=20) and W-MTA (n=20) will be implanted into subcutaneous rats. In the control group GC (n=20), empty tubes will be implanted. After 7, 15, 30 and 60 days, the rats will be sacrificed with anesthetic overdose and the implanted tubes surrounded by connective tissue will be also removed and will be fixed. After fixation, the specimens will be dehydrated, treated with xylene and embedded in paraffin. To evaluate the tissue reaction promoted by materials implanted into subcutaneous, from HE-stained sections the number of inflammatory cells and fibroblasts, and the thickness of the capsules will be obtained. The birefringent collagen content will be quantified from sections subjected to the picrosirius-red and analyzed under a light microscope with polarized filters. Interleukin-6 (IL-6), a pro-inflammatory cytokine, will be detected by immunohistochemical reaction and the number of immunolabelled cells will be computed. The bioactive potential will be evaluated by von Kossa reaction and analysis of unstained sections analyzed under polarized light, which will be performed to detect calcium deposits and calcite crystals, respectively. Some sections, after von Kossa reaction, will be subjected to immunohistochemistry for the detection of alkaline phosphatase, an enzyme produced by mineralized tissue cells. Data will be analyzed by two-way ANOVA and Tukey post-test (pd0.05).
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