The effects of smoking on the bone remodeling process: a study in patients submitted to total hip arthroplasty

• Grant number: 19/21818-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2020
• End date: December 31, 2021
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Fernanda Degobbi Tenorio Quirino dos Santos Lopes
• Grantee: Alex Ferreira da Silva
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Many clinical studies demonstrate the impact of smoking on increased bone fragility, but most of these studies are restricted to imaging and symptom analysis of patients that make it difficult to advance the greater impact of pathophysiology involved in the repair process and tissues injury. Thus, to understand the role of different cell types, chemokines and growth factors in the bone tissue remodeling process, patients tissues will be collected from total hip arthroplasty to evaluate chemokines and growth factors involved in activity of these cells to maintain the bone tissue repair and tissue histoarchitecture. Thus, patients will be divided into three experimental groups: Smokers (patients who have smoke before surgery), elective smokers (patients who smoke stop smoking more than 6 weeks before surgery) and non-smokers (patients who had never smoke). The bone tissue samples will be homogenized as chemokines used directly in technical repair metabolism such as RANKL, OPG, IL-1, IL-6, TNF-alpha by ELISA. Specific immunohistochemical markings will be made on bone tissue to quantify osteoblasts and osteoclasts and members of the organic matrix such as type I and V collagen. (AU)