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Influence of zinc Cooper-doped 58S bio-glass embedded membranes on osteogenesis: in vitro study

Grant number: 19/25744-9
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2020
Effective date (End): December 31, 2020
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Luana Marotta Reis de Vasconcellos
Grantee:Marina Santos Fernandes
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil


Due the high demand for biomaterials that assist in bone repair, it is necessary todevelop new technologies to help repair bone structure problems and improve thepopulation's quality of life. The aim of this study will be to evaluate the influence ofelectroplated ZS-58 (Zn) and copper (Cu) doped 58S bio-glass PCL membranes onthe activity and differentiation of mesenchymal cells obtained from rat femurs. Aftercell isolation and cell confluence, 2x104 cell plating per well will be performed, whichwill previously receive the biomaterials that will be divided into different groups: a)group P: mesenchymal cells plated with PCL, b) group PB: mesenchymal cells platedwith the PCL / Bioglass composite, c) PBC group: mesenchymal cells plated with theCu-doped PCL / Bioglass composite, d) PBZ group: mesenchymal cells plated withthe Zn doped PCL / Bioglass composite. There will also be group C: cellular control -mesenchymal cells plated without biomaterial. All these groups will be analyzed withplating in supplemented culture medium and osteogenic culture medium. Prior to thein vitro stage, the morphology of the biomaterial will be analyzed by scanningelectron microscopy (SEM). Subsequently, biological cytotoxicity tests will beperformed by MTT colorimetric test, evaluation of total protein content, alkalinephosphatase activity and formation of mineralization nodules. The data will bestatistically analyzed, initially through the normality test aiming at the application ofthe appropriate statistical test. The significance level adopted will be the conventionalvalue of 5%.

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