Influence of the brushing technique and the concentration of the toothbrushing slurry on enamel erosive wear

• Grant number: 19/20126-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2020
• End date: December 31, 2020
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Taís Scaramucci Forlin
• Grantee: Ana Luísa Meira Renzo
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

This study aims to evaluate the influence of different brushingtechniques and different concentrations of dentifrice / saliva suspensions onthe progression of erosive wear in bovine enamel. Whole bovine incisors willbe used, from which 4mm x 4mm x 2mm enamel specimens will be obtainedfrom their crowns. The specimens will be included in acrylic resin, flattenedand polished. After the planning step, the specimens will be subjected to aninitial profile analysis using an optical profilometer. Then an adhesive tapewill be placed on the surface of these fragments, leaving a 4mm x 1mmwindow exposed to subsequent testing. The specimens will be randomlydistributed into 12 experimental groups, according to the 2 experimentalfactors: 1) Brushing movement, in 4 levels (horizontal, zig-zag, circular andelliptical) and 2) Concentration of toothpaste / saliva suspension, in 3 levels (1part toothpaste: 2 parts artificial saliva; 1 part toothpaste: 3 parts artificialsaliva; 1 part toothpaste: 4 parts artificial saliva). The specimens will besubjected to an erosive-abrasive cycling, which will consist of 5 minimmersion in 1% citric acid solution, followed by 60 min exposure to artificialsaliva. This step will be repeated 4 times a day for 5 days. After the first andlast erosive challenges, the specimens will be exposed to brushing accordingto each experimental group for 15s and 150 g load. The specimens will beimmersed during brushing in 30 ml of the suspensions, which will be preparedaccording to the experimental groups using a fluoride dentifrice (NaF), andwill remain so until 2 min of exposure to the suspension is completed . At theend of cycling, the adhesive strips will be removed and the surface loss ofspecimens determined with an optical profilometer. The data obtained will bestatistically analyzed, considering a significance level of 5%. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAKAE, LETICIA OBA; RENZO, ANA LUISA MEIRA; VIANA, ITALLO EMIDIO LIRA; NIEMEYER, SAMIRA HELENA; CARVALHO, THIAGO SAADS; SCARAMUCCI, TAIS. Impact of different brushing/abrasion protocols on erosive tooth wear for in vitro studies. ARCHIVES OF ORAL BIOLOGY, v. 148, p. 4-pg., 2023-02-22. (20/05374-0, 19/20126-5)