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In situ evaluation of the anti-erosive effect of the association between sodium fluoride and propylene glycol alginate

Grant number: 17/09408-3
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2018
Effective date (End): March 31, 2019
Field of knowledge:Health Sciences - Dentistry
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Taís Scaramucci Forlin
Grantee:Letícia Oba Sakae
Home Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The aim of this study is to evaluate the anti-erosive effect of solutions contaning sodium fluoride (F: 225 ppm F), sodium fluoride + stannous chloride (F+Sn: 225 ppm F + 800 ppm Sn), sodium fluoride + propylene glycol alginate (F+PGA: 225 ppm F + 0,1% PGA), and deionized water (negative control), using a 4-phase, split mouth, single bind, cross over in situ trial. Twelve subjects will participate in the study after signing a written informed consent. In each study phase, the subjects will use removable mandible devices containing 4 enamel specimens, which will submitted to an erosion-remineralization-abrasion or erosion-remineralization cycling model (split mouth) of 5 days. The erosion-remineralization-abrasion cycling will consist of 5 min extra-oral immersion in 1% citric acid (pH=2.6), followed by 120 min remineralization in situ. This procedure will be repeated 4x/day. Treatment with the test solutions will be performed 2x/day, after the first and the last erosive challenges. Before treatment, toothbrushing will be performed with an electric toothbrush and a dentifrice slurry (1450 ppm F) for 5 s, in a total of 2 min exposure to the slurry. or the erosion-remineralization cycling, there will only 2 min exposure to the slurry, without brushing. At the end, enamel surface loss (in µm) will be evaluated with optical profilometry. Surface loss data will be analyzed with ANOVA suitable for crossover studies and Tukey, or Krukal-wallis and Tukey, depending on the normality and homoscedasticity distribution of the data. The significance level will be set at 5%. (AU)