Effects of photobiomodulation short-term on the pancreas of diabetic rats

• Grant number: 19/19851-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2020
• End date: February 28, 2022
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal Investigator: Ana Claudia Muniz Renno
• Grantee: Adriane Marie Angri Burek
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil

Abstract

Type 1 diabetes mellitus (DM1) is a chronic metabolic disease characterized by insufficient insulin secretion due to autoimmune destruction of pancreatic ² cells. In order to study this disease, the streptozotocin (STZ) induction model that leads to specific pancreatic ²-cell necrosis is often used. For the purpose of to provide a therapeutic strategy for DM, we find in photobiomodulation therapy (PBM) a promising potential promoting regeneration and survival of pancreatic ² cells. Thus, the aim of the present study is to evaluate the effects of two acutely applied PBM protocols on pancreatic regeneration, and insulin and glucose tolerance serological parameters in an experimental DM model in diabetic rats. For this, 40 male three-month-old Wistar rats will be used, which will be randomly distributed into 5 groups: healthy animals (CG), untreated DM control group (DG), animals with DM treated group with PBM 30 J/cm² (L30), animals with DM treated with 90 J/cm² (L90) and healthy animals treated with 90 J/cm² (HL90). After anesthesia with anesthetic overdose, a dose of 60 mg/kg STZ will be applied to the penile vein to induce DM1. After 15 days of disease induction, the animals' glycemia will be verified to confirm DM1. For the next 3 days, the animals will be submitted to PBM according to parameters of each group. After the last day of PBM the glucose and insulin tolerance in the animals will be evaluated. Immediately after euthanasia, the pancreas will be surgically dissected for histological processing (HE), for histopathological and morphometric analysis of pancreatic islets and e analysis oh the anti-insulin and 8-OhdG proteins. In addition, will be evaluated Aconitase protein expression by Western Blot. For statistical analysis will be used ANOVA. (AU)