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Platinum complex functioned to gold nanoparticles for tumor treatment

Grant number: 20/06576-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2020
Effective date (End): August 31, 2021
Field of knowledge:Engineering - Biomedical Engineering
Principal Investigator:Leandro José Raniero
Grantee:Vitor Gabriel Poli de Lima
Home Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil

Abstract

Platinum complexes have often been used in the development of anticancer drugs due to their ability to bind to DNA nucleobases, specifically guanine, and cleave these molecules, preventing their replication and transcription, causing cell death by apoptosis. Nanotechnology has been one of the great tools used in several areas of science due to its applicability and versatility, this factor being the result of the unique and exclusive properties of materials on a nanometric scale. Gold nanoparticles are one of the nanometric materials of greatest interest in the elaboration of nanomedical complexes because they have chemical and optical characteristics dependent on their size and shape, in addition to their high biocompatibility and drug loading property, which can make treatment more direct and selective. For the production of nanoparticles functionalized with the platinum complex, thiolated oligonucleotides will be used as intermediate ligands, with the bond between the sulfur present at one end and the gold nanoparticle formed due to the affinity between the elements, in the same way as platinum it will be linked to the guanines present in the chains of the thiolated oligonucleotides used, namely: SC-4GAAGGA and SC-4GAAAAG. The amount of platinum used to form the complex will be determined from a variation in concentration of the same added during the process of synthesis of the structure, just as the solution will have its pH varied to study the interference of this characteristic of the solution in the formation of the complex, being confirmed the functionalization from FT-IR Spectroscopy, in addition to the stability of particles pre and post functionalization to be analyzed by UV-Visible Spectroscopy, DLS and Zeta Potential, as well as stability tests with saline solutions.