Applications of non-thermal atmospheric plasmas in Dentistry: from the bench to the clinic

• Grant number: 20/09481-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2020
• End date: May 31, 2025
• Field of knowledge: Physical Sciences and Mathematics - Physics - Physics of Fluids, Plasma Physics and Electrical Discharge
• Principal Investigator: Konstantin Georgiev Kostov
• Grantee: Fellype do Nascimento
• Host Institution: Faculdade de Engenharia (FEG). Universidade Estadual Paulista (UNESP). Campus de Guaratinguetá. Guaratinguetá , SP, Brazil
• Associated research grant: 19/05856-7 - Use of low temperature atmospheric pressure plasma in dentistry: from laboratory bench to clinics, AP.TEM
• Associated scholarship(s): 21/14391-8 - Advanced characterization of portable atmospheric pressure plasma jet devices especially for applications in medicine and dentistry, BE.EP.PD

Abstract

This proposal aims to investigate the applications of cold plasma at atmospheric pressure (Low Temperature Atmospheric Pressure Plasma - LTAPP) in Dentistry. The general objective is to develop protocols for the application of plasma and plasma activated liquid in vivo and ex vivo for diseases of dental interest and portable equipment for clinical application. As specific objectives it is intended to develop effective protocols for the following conditions of dental interest: oral candidiasis, periodontitis, dental caries, endodontic infection and oral mucositis. Protocols for direct application of plasma or plasma-treated liquid (LTP, indirect application) will be used. In the case of infectious diseases, the effective LTAPP parameters for each specific pathogenic biofilm will initially be standardized in vitro. Subsequently, these parameters will be evaluated for cyto and genotoxicity, adopting the most appropriate cell line for each case. The effective (maximum antimicrobial effect) and safe (less toxicity) protocols will be applied in in vivo or ex vivo models for validation. Regarding mucositis, protocols using direct or indirect application will be evaluated in rats treated with 5-fluorouracil, followed by histological and immunohistochemical analyzes. The systemic toxicity of LTP will be evaluated in a model of Drosophila melanogaster. The plasma generating equipment used for this study was developed and validated by the project team, with several associated publications. The current equipment is laboratory bench sized. Thus, it is also the objective of this project to transpose the current equipment into a small and portable prototype, maintaining its potential. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NASCIMENTO, FELLYPE; PETROSKI, KLEBER; KOSTOV, KONSTANTIN. Effects of O-2 Addition on the Discharge Parameters and Production of Reactive Species of a Transferred Atmospheric Pressure Plasma Jet. APPLIED SCIENCES-BASEL, v. 11, n. 14, JUL 2021. (20/09481-5, 19/05856-7)

KOGA-ITO, CRISTIANE YUMI; KOSTOV, K. G.; MIRANDA, F. S.; MILHAN, N. V. M.; NETO, N. F. AZEVEDO; NASCIMENTO, F.; PESSOA, R. S.. Cold Atmospheric Plasma as a Therapeutic Tool in Medicine and Dentistry. PLASMA CHEMISTRY AND PLASMA PROCESSING, v. N/A, p. 37-pg., 2023-08-27. (16/07196-6, 21/14181-3, 23/02268-2, 19/05856-7, 20/09481-5)

DO NASCIMENTO, FELLYPE; STANCAMPIANO, AUGUSTO; TREBULOVA, KRISTINA; DOZIAS, SEBASTIEN; HRUDKA, JAN; KRCMA, FRANTISEK; POUVESLE, JEAN-MICHEL; KOSTOV, KONSTANTIN GEORGIEV; ROBERT, ERIC. Plasma Electrode Dielectric Barrier Discharge: Development, Characterization and Preliminary Assessment for Large Surface Decontamination. PLASMA CHEMISTRY AND PLASMA PROCESSING, v. 43, n. 6, p. 27-pg., 2023-10-25. (20/09481-5, 21/14391-8, 19/05856-7)

SAMPAIO, ALINE DA GRACA; MILHAN, NOALA VICENSOTO MOREIRA; DO NASCIMENTO, FELLYPE; KOSTOV, KONSTANTIN GEORGIEV; KOGA-ITO, CRISTIANE YUMI. Cold Atmospheric Pressure Plasma May Prevent Oral Mucositis-Related Candidemia in Chemotherapy-Treated Rats. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 25, n. 21, p. 15-pg., 2024-11-01. (20/09481-5, 22/04723-6, 19/25652-7, 19/05856-7, 21/00046-7)

DO NASCIMENTO, FELLYPE; LEAL, BRUNO SILVA; QUADE, ANTJE; KOSTOV, KONSTANTIN GEORGIEV. Different Radial Modification Profiles Observed on APPJ-Treated Polypropylene Surfaces according to the Distance between Plasma Outlet and Target. POLYMERS, v. 14, n. 21, p. 17-pg., 2022-11-01. (20/09481-5, 19/05856-7, 21/14391-8)

KOSTOV, KONSTANTIN G.; BARBOSA, ANANIAS A.; DO NASCIMENTO, FELLYPE; CARDOSO, PAULO F. G.; ALMEIDA, ANA C. P. L.; QUADE, ANTJE; LEGENDRE, DANIEL; HEIN, LUIZ R. O.; SILVA, DIEGO M.; KOGA-ITO, CRISTIANE Y.. Plasma Surface Modification of the Inner Wall of Montgomery's Tracheal Implant (T-Tube). POLYMERS, v. 16, n. 22, p. 17-pg., 2024-11-01. (21/02680-5, 19/05856-7, 20/09481-5, 23/02436-2)

DO NASCIMENTO, FELLYPE; PETROSKI, KLEBER A.; NISHIME, THALITA M. C.; KOSTOV, KONSTANTIN G.. Measurements of power dissipated in an atmospheric pressure plasma jet device with double plasma discharge ignition. EUROPEAN PHYSICAL JOURNAL D, v. 78, n. 12, p. 17-pg., 2024-12-01. (20/09481-5, 19/05856-7)

DO NASCIMENTO, FELLYPE; KOSTOV, KONSTANTIN GEORGIEV; MACHIDA, MUNEMASA; FLACKER, ALEXANDER. Properties of DBD Plasma Jets Using Powered Electrode With and Without Contact With the Plasma. IEEE TRANSACTIONS ON PLASMA SCIENCE, v. 49, n. 4, p. 1293-1301, APR 2021. (20/09481-5)

DO NASCIMENTO, FELLYPE; QUADE, ANTJE; CANESQUI, MARA ADRIANA; KOSTOV, KONSTANTIN GEORGIEV. Different configurations of transferred atmospheric pressure plasma jet and their application to polymer treatment. CONTRIBUTIONS TO PLASMA PHYSICS, v. 63, n. 1, p. 14-pg., 2022-10-11. (20/09481-5, 19/05856-7)