Evaluation of the mechanisms of action of Bacillus thuringiensis (Bacillales: Bacillaceae) encapsulated in polymeric microparticles

• Grant number: 20/12779-6
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: May 01, 2021
• End date: April 30, 2022
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Ricardo Antonio Polanczyk
• Grantee: Jhones Luiz de Oliveira
• Supervisor: Alejandra Bravo
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Institution abroad: Universidad Nacional Autónoma de México, Morelos (UNAM), Mexico
• Associated to the scholarship: 18/21142-1 - Encapsulation of Bacillus thuringiensis (Bacillales: Bacillaceae) in polymeric microparticles and association with nanoencapsulated chemical agent: evaluation of biological activity and mechanisms of action, BP.PD

Abstract

Bacillus thuringiensis (Bt) bacteria have been recognized for their high potential in the control of various agricultural pests. Recently, there has been multiple advances in both, the research and the market of novel insecticidal products, involving this biological agent. Developments in micro/nanotechnology have opened new perspectives for the production of more efficient formulations that can overcome some obstacles associated with the use of Bt-products in the field, such as formulation instability and loss of activity due to the degradation of toxins by their exposure to ultraviolet radiation, among other problems. The present proposal will explore the efficiency and mechanism of action of Bt-products based on micro/nanotechnology formulations. Several strategies will be addressed in order to assess the influence of encapsulation on the action of Bt toxins, such as analysis of crystals solubilization and activation in the gastric juice of the larvae, as well as their biding to specific receptors. In addition, the damage caused by microencapsulated Bt in the larval intestinal epithelium will also be investigated in vivo. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

ABRANTES, DANIELE CARVALHO; ROGERIO, CAROLINA BARBARA; DE OLIVEIRA, JHONES L.; CAMPOS, ESTEFANIA V. R.; DE ARAUJO, DANIELE RIBEIRO; PAMPANA, LAURINDO CESAR; DUARTE, MARCELO JOSE; VALADARES, GEORGIO FREESZ; FRACETO, LEONARDO FERNANDES. Development of a Mosquito Repellent Formulation Based on Nanostructured Lipid Carriers. FRONTIERS IN PHARMACOLOGY, v. 12, OCT 11 2021. (18/02404-5, 17/24402-1, 18/14734-0, 20/12779-6)

PEREIRA, ANDERSON DO ESPIRITO SANTO; DE OLIVEIRA, JHONES LUIZ; SAVASSA, SUSILAINE MAIRA; ROGERIO, CAROLINA BARBARA; DE MEDEIROS, GERSON ARAUJO; FRACETO, LEONARDO FERNANDES. Lignin nanoparticles: New insights for a sustainable agriculture. JOURNAL OF CLEANER PRODUCTION, v. 345, p. 16-pg., 2022-04-15. (17/21004-5, 18/21142-1, 20/12779-6, 20/05415-8)

DE OLIVEIRA, JHONES L.; FRACETO, LEONARDO FERNANDES; BRAVO, ALEJANDRA; POLANCZYK, RICARDO ANTONIO. Encapsulation Strategies for Bacillus thuringiensis: From Now to the Future. Journal of Agricultural and Food Chemistry, v. 69, n. 16, p. 4564-4577, APR 28 2021. (20/12779-6, 18/21142-1, 17/21004-5)