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Interrogation of a potential transcription regulation in Trypanosoma cruzi by evaluation of nascent transcripts genome-widely

Grant number: 21/03219-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): July 01, 2021
Effective date (End): June 30, 2023
Field of knowledge:Biological Sciences - Parasitology - Protozoology of Parasites
Principal researcher:Julia Pinheiro Chagas da Cunha
Grantee:Natália Karla Bellini
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Associated research grant:18/15553-9 - Going deeper into Trypanosoma cruzi chromatin regulation: identifying new players and quizzing its impact on a potential transcription control, AP.JP2


In trypanosomes, it is general accepted that gene expression is mainly regulated by post transcriptional mechanisms due to the lack of classical RNA Pol II promoters regions and policistronic transcription. However, previous low-scale run-on experiments indicate that satellites genes have lower expression, and a significant decrease in global transcription is observed when replicative forms are compared to non-replicative forms (ie. Metacyclics) suggesting some layer of transcription regulation (Elias, Marques-Porto et al. 2001, Ferreira, Dossin Fde et al. 2008). In addition, it was observed in Leishmania (genus of Trypanosomatid order) that genes associated to cell cycle can be regulated at transcription level (Chandra, Yadav et al. 2017). Thus, here we want to investigate, in a genome-wide scale, the transcription rate of all genomic regions of T.cruzi. To that, we will perform Global Run-on sequencing (GRO-seq) followed by an intense computational analysis including the integration with public datasets. Nascent transcripts analysis will also be expanded to interrogate a potential transcription regulation along EàM differentiation and during cell cycle using some selected genomic regions detected at genome-wide analysis. This project requires both an intense wet- and dry-lab efforts to reach their full potential; therefore, postdoctoral students with a previous background on both training will be preferable. (AU)

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