Injuries to the motor nerve by injection needle or needle suture: experimental study in the rat

• Grant number: 21/04352-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2021
• End date: October 31, 2022
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Fausto Viterbo de Oliveira Neto
• Grantee: Matheus Scuracchio Fernandes
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

When performing invasive procedures, which as a rule involve needles, there is a chance of injuring a peripheral nerve. Any anesthesia, suture, blockage, infiltration, etc. is subject to that risk. Thus, a way of experimentally simulating these situations was sought. Objective: It will be possible to evaluate possible peripheral nerve damage caused by the entry and exit of an injection needle, by transfixing the nerve through a suture point, by injecting saline solution [saline solution (0.9% NaCl)] inside of the nerve and by the injection of lidocaine into the nerve. Method: It consists of randomly separating 30 Wistar rats in 5 groups with 6 animals (Control Group, Needle Group, Suture Group, Serum Group, and Lidocaine Group). In the control group, nothing will be done. In the Agulha Group, the animals will be anesthetized and the fibular nerve will be transfixed by an insulin needle for 5 minutes. In the Suture Group, the animals will be anesthetized and the fibular nerve will be transfixed by a suture stitch that will be removed after 48 hours. In the Serum Group, the animals will be anesthetized and an intraneural injection of saline will be made in the fibular nerve. In the Lidocaine Group, the animals will be anesthetized and an intraneural injection of 1% lidocaine + epinephrine will be performed. Data Analysis: The animals will be evaluated by the "walking test" - WTA (Walking Track Analysis) for 2 months, after this period they will undergo electrophysiological studies and the muscles will be submitted to a strength test. After euthanasia, bilateral fibular nerves and bilateral cranial tibial muscles will be extracted and sent for histological analysis. Statistical Analysis: the WTA results will go through the profile analysis, the other results will be evaluated by the Tukey method for multiple comparisons between the group means. If the variables do not have a normal distribution, non-parametric tests such as Kruskal-Wallis and Friedman will be used. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, MATHEUS SCURACCHIO; VITERBO, FAUSTO; RODRIGUES, LORENA DELGADO; DI LUCCAS, CAIO BORGES; NETO, BALDUINO FERREIRA DE MENEZES. Nerve Injury and Peripheral Nerve Functional Loss From Injection and Suture Needles: An Experimental Study on Rats. AESTHETIC SURGERY JOURNAL, v. N/A, p. 7-pg., 2023-08-22. (21/04352-5, 21/04429-8)