Research Grants 23/12881-3 - Cirurgia parendodôntica, Obturação retrógrada - BV FAPESP
Advanced search
Start date
Betweenand

Development and Analysis of a new hydraulic repair cement containing Fibrin Sealant

Abstract

Fibrin sealant is a material used in the medical field for over 70 years with the aim of assisting in the process of repairing nerve damage, assisting in suturing medication, aiding in hemostasis and repairing skin wounds. This material is known not only for its repair potential, but also for its versatile use in various areas, including Dentistry. Fibrin sealant can be obtained from autologous or homologous derivatives, however, homologues have been the most used. The Fibrin sealant developed by the Center for the Study of Poisons and Venomous Animals (CEVAP) is composed derivative (Gyroxin -Thrombin-like) obtained from the Rattlesnake snake (Crotalus Durissus Terrificus), fibrinogen (Factor VIII, XIII, Von Willebrand Factor and Fibronectin) extracted from a large animal (Buffalo) and Calcium Chloride. Therefore, the objective of this work is to develop a hydraulic repair cement and analyze its physical-chemical and biological properties (cytotoxicity) and compare this cement to other cements on the market used for the same purpose (MTA Repair HP, Bio-C Repair and Sealer 26 ). The experimental repair cement will be evaluated in two proportions and two types of liquid, which were obtained in a previous pilot study, being 3.5 grams and 3 grams of powder (40% Di and Tri Calcium Silicate, 3% Calcium Chloride Calcium, 2% Calcium Oxide and 55% Bismuth Subcarbonate). The liquid will be a total of 1 mL, being 50% fibrinogen, 25% Calcium Chloride and 25% Gyroxin or 100% Gyroxin. 50 prototypes of upper central incisors printed in transparent resin (n=10) will be used, which will have the canals instrumented up to a Reciproc R50 (50.05) and filled using the single cone technique with a 50.05 cone and Sealer Plus cement. Subsequently, the apical 3 mm will be apicectomized and a 3 mm deep retrograde cavity will be created using an ultrasonic insert. Then the cements will be inserted into the cavities and the teeth will be fixed individually in an acrylic bottle with the apical portion submerged in 13 mL of ultrapure water and stored in a humid study. The pH will be measured at periods of 3, 24, 72 and 168 hours. Immediately after immersion, the teeth will be scanned in Micro-CT (1174) to obtain the initial volume of retrograde filling material and, subsequently, scanned at periods 24, 72 and 168 hours to evaluate solubility using the same parameters. In addition, the initial and final setting time test will be carried out with the respective cements using Gilmore needles (113.5 and 456.5 grams). The hydraulic cements (experimental cement 01, experimental cement 02, Bio-C Repair and MTA Repair HP) will be inserted into a wet plaster disk, while the Sealer 26 cement into a metal ring of the same dimensions. The radiopacity of the cements used in this study will be carried out using a metal ring with an internal diameter of 5 mm and a thickness of 1 mm (n=3). Then, after filling the rings and positioning them on an occlusal film, radiographs were taken for later analysis using Adobe Photoshop Software. Finally, the cytotoxicity of the cements will be evaluated using cells from the MC3T#-E1 (ATCC®) communion pre-odontoblastic cell line. The cements used in this study will be evaluated at a concentration of 0.1 g/mL (100%) in ±-MEM medium, we will begin serial dilution at concentrations of 10%, 1%, 0.1% and 0.01%. The samples will be incubated in an oven at 37°C for 24 hours. The experiment will be carried out as recommended by ISO-10993-5 standards. The data obtained will be tabulated for subsequent statistical analysis. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
More itemsLess items
Articles published in other media outlets ( ):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Please report errors in scientific publications list using this form.
X

Report errors in this page


Error details: