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Development and analysis of two cements for use in retrograde filling and sealing of perforations

Grant number: 17/06545-0
Support type:Scholarships in Brazil - Master
Effective date (Start): November 01, 2017
Effective date (End): July 31, 2019
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal researcher:Marco Antonio Hungaro Duarte
Grantee:Pedro Cesar Gomes Titato
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The objective of this study is to develop and analyze the physical-chemical properties and dental colors change of two retro-obturator experimental cements and its uses in cases of perforations and retrofilling, one consisting of calcium silicate and calcium phosphate and another associating silica tricalcium silicate, containing different radiopacifying agents and finally compare to the properties of MTA HP and MTA White Angelus. The materials will be divided into 6 groups: I - MTA White; II - HP MTA; III - Experimental 1 with Powder (60% Calcium Silicate, 10% Calcium Phosphate, 20% Zirconium Oxide) and Liquid (80% water and 20% Arnica glycolic extract); IV -Experimental 2 with Powder (60% Calcium Silicate, 10% Calcium Phosphate, 20% Calcium Tungstate) and Liquid (80% water and 20% Arnica glycolic extract); V -Experimental 3 with Silicone Cement + 20% (wt) of tricalcium silicate + 10% (wt) Zirconium Oxide and VI - Experimental 4 with Silicone Cement + 20% (wt) of tricalcium silicate + 10% By weight) of calcium tungstate. For tests of radiopacity, film thickness, flow, ISO 6876/2001 specifications shall be followed. The ASTM C266 / 2008 standard will be used to determine the prey time. For determination of the release of calcium ions, an atomic absorption spectrophotometer will be used. For the determination of the pH will be carried out by means of a pH meter previously calibrated with solutions of known pHs (4, 7 and 14). The volumetric change will be analyzed using MicroCT. To evaluate the tooth color change there's going to be used 10 bovine teeth for each group. The teeth will have the pulp chambers clean and clogged with the cements. After the periods of 7, 15, 30 and 60 days, the teeth will be submitted to spectrophotometry. The results will be submitted to D'Agostino and Pearson tests for normal distribution verification. If normal, the parametric ANOVA test will be used. In case of absence of normality, the non-parametric Kruskal-Wallis test will be used. For all tests the significance level of 5% will be considered. (AU)

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