Effect of the use of Limonene associated with bioactive glass-ceramic on dentin/adhesive interface

• Grant number: 21/04598-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2021
• End date: August 31, 2022
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Fernanda de Carvalho Panzeri
• Grantee: Arthur Henrique Garcia Pinto
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

This study aims to evaluate the effect of the use of the extract of limonene associated with Biosilicate on bond strength to dentin. A pilot study was conducted to determine the most efficient concentration and time of application of limonene. Thus, extracts of limonene were tested at 0.5% and 1%, applied on the dentin surface for 30 seconds or 1 minute with a microbrush. The substrate was characterized by scanning electron microscopy (SEM, EVO MA10, ZEISS). SEM images demonstrated that the application of the extract at a concentration of 0.5% with a microbrush actively for 30 seconds resulted in a more defined and uniform dentin. This protocol will be used to verify the adhesive/dentin bond strength. For this, 80 healthy human molars will be selected and occlusal cavities (5 mm x 4 mm x 4 mm) will be prepared using carbide burs. Then, all the teeth will be randomly separated into 4 groups (n =20), according to the treatment received before the adhesive system (Single Bond Universal, 3M ESPE): Control Group - Adhesive System; LIM Group - Limonene Extract; Bio Group - 10% Biosilicate suspension; LIMBio Group - LIM + Bio. After the restorative procedures (Filtek Z350XT, 3M ESPE), the samples will be sectioned into sticks, separated, and stored in distilled water at 37 ° C for 24 hours and 6 months. After those storage periods, they will be subjected to the microtensile test (0,5 mm/min). The data will be analyzed according to the most appropriate statistical test. The fracture patterns will be observed with a digital microscope (VH-M100, Keyence) and the adhesive interface through SEM. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PINTO, ARTHUR HENRIQUE GARCIA; GENG-VIVANCO, ROCIO; PIRES-DE-SOUZA, FERNANDA DE CARVALHO PANZERI. Effect of limonene associated with bioactive glass-ceramic on dentin/adhesive interface. European Journal of Oral Sciences, v. N/A, p. 8-pg., 2023-07-27. (21/04598-4)