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Effect of the use of Limonene associated with bioactive glass-ceramic on dentin/adhesive interface

Grant number: 21/04598-4
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2021
Effective date (End): August 31, 2022
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Fernanda de Carvalho Panzeri Pires de Souza
Grantee:Arthur Henrique Garcia Pinto
Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


This study aims to evaluate the effect of the use of the extract of limonene associated with Biosilicate on bond strength to dentin. A pilot study was conducted to determine the most efficient concentration and time of application of limonene. Thus, extracts of limonene were tested at 0.5% and 1%, applied on the dentin surface for 30 seconds or 1 minute with a microbrush. The substrate was characterized by scanning electron microscopy (SEM, EVO MA10, ZEISS). SEM images demonstrated that the application of the extract at a concentration of 0.5% with a microbrush actively for 30 seconds resulted in a more defined and uniform dentin. This protocol will be used to verify the adhesive/dentin bond strength. For this, 80 healthy human molars will be selected and occlusal cavities (5 mm x 4 mm x 4 mm) will be prepared using carbide burs. Then, all the teeth will be randomly separated into 4 groups (n =20), according to the treatment received before the adhesive system (Single Bond Universal, 3M ESPE): Control Group - Adhesive System; LIM Group - Limonene Extract; Bio Group - 10% Biosilicate suspension; LIMBio Group - LIM + Bio. After the restorative procedures (Filtek Z350XT, 3M ESPE), the samples will be sectioned into sticks, separated, and stored in distilled water at 37 ° C for 24 hours and 6 months. After those storage periods, they will be subjected to the microtensile test (0,5 mm/min). The data will be analyzed according to the most appropriate statistical test. The fracture patterns will be observed with a digital microscope (VH-M100, Keyence) and the adhesive interface through SEM. (AU)

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