Tissue-specific modulation of the proteome, phytoquelatin synthesis, and activity of antioxidant enzymes of tomato in response to Cd, by using reciprocal grafting

• Grant number: 20/12666-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2021
• End date: December 31, 2025
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Ricardo Antunes de Azevedo
• Grantee: Deyvid Novaes Marques
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 22/11018-7 - Metabolomics for cadmium stress response: focus on grafted tomato plants, BE.EP.PD

Abstract

Cadmium (Cd) is a heavy metal whose concentration has been increased considerably over the last few decades in several countries (which is related to anthropogenic activities). This metal is potentially harmful to human health and plants, being one of the most threatening contaminants to the environment. However, there are very few studies whose approach is the development of management strategies to reduce the Cd contamination of edible parts of plants, in line with the understanding about the dissection of the role of root and shoot to the observed tolerance pattern, as well as on related endogenous mechanisms. In such a context, this work aims to study the tissue-specific modulation of the tomato proteome in response to Cd by using reciprocal grafting. We will use 36 grafted tomato genotypes (a number that has never before been used in studies published in high-impact scientific journals), in order to evaluate the combination of genotypes previously characterized as being contrasting when it comes to tolerance to Cd. For this purpose, label-free quantification-based proteomics will be conducted, according to the scholarship applicant's knowledge acquired during his doctoral internship at the Max Planck Institute (Max Planck Society; Max Planck Institute for Plant Breeding Research, Germany). Recently, we have obtained two international publications, involving phytoquelatins (important molecules involved in modulating the Cd accumulation and tolerance in plants) and antioxidant responses of plants exposed to Cd. In the present study, the quantification of PCs and analysis of the activity of antioxidant enzymes also will be studied. Through the large number of grafted tomato genotypes whose samples were previously collected (FAPESP, Process number 2018/22671- Regular Research Grant), and a large amount of data generated by large-scale proteomic analysis (which will consider all proteins present in cells of tomato plants and a far larger number of identified proteins compared to conventional gel-based proteomic analyzes), we will conduct an unprecedented and high impact study, serving as a model for tomato and cultivated species. The results will provide important information on the contribution of each tomato genotype used as shoot and rootstock to the general tolerance pattern of each grafted plant and to consolidate management strategies to reduce or avoid Cd contamination of edible parts of plants. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARQUES, DEYVID NOVAES; MASON, CHASE; STOLZE, SARA CHRISTINA; HARZEN, ANNE; NAKAGAMI, HIROFUMI; SKIRYCZ, ALEKSANDRA; PIOTTO, FERNANDO ANGELO; AZEVEDO, RICARDO ANTUNES. Grafting systems for plant cadmium research: Insights for basic plant physiology and applied mitigation. Science of The Total Environment, v. 892, p. 18-pg., 2023-06-09. (22/11018-7, 21/06117-3, 20/12666-7, 17/05544-0, 18/22671-8, 18/20706-9)

MARQUES, DEYVID NOVAES; NOGUEIRA, MARINA LIMA; GAZIOLA, SALETE APARECIDA; BATAGIN-PIOTTO, KATHERINE DERLENE; FREITAS, NATALIA CHAGAS; ALCANTARA, BERENICE KUSSUMOTO; PAIVA, LUCIANO VILELA; MASON, CHASE; PIOTTO, FERNANDO ANGELO; AZEVEDO, RICARDO ANTUNES. New insights into cadmium tolerance and accumulation in tomato: Dissecting root and shoot responses using cross-genotype grafting. Environmental Research, v. 216, p. 14-pg., 2023-01-01. (20/12666-7, 22/11018-7, 17/05544-0, 18/20706-9, 21/06117-3, 16/05788-3, 16/14349-3)

MARQUES, DEYVID NOVAES; THIENGO, CASSIO CARLETTE; AZEVEDO, RICARDO ANTUNES. Phytochelatins and Cadmium Mitigation: Harnessing Genetic Avenues for Plant Functional Manipulation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 26, n. 10, p. 20-pg., 2025-05-16. (20/12666-7, 22/11018-7)

DE SOUZA, CLAUDIA REGINA BATISTA; SERRAO, CLEYSON PANTOJA; BARROS, NICOLLE LOUISE FERREIRA; DOS REIS, SAVIO PINHO; MARQUES, DEYVID NOVAES. Plant bZIP Proteins: Potential use in Agriculture - A Review. CURRENT PROTEIN & PEPTIDE SCIENCE, v. 25, n. 2, p. 13-pg., 2024-01-01. (20/12666-7, 22/11018-7, 17/05544-0, 18/20706-9)

MARQUES, DEYVID NOVAES; PIOTTO, FERNANDO ANGELO; AZEVEDO, RICARDO ANTUNES. Phosphoproteomics: Advances in Research on Cadmium-Exposed Plants. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 25, n. 22, p. 14-pg., 2024-11-01. (20/12666-7, 22/11018-7)

MARQUES, DEYVID NOVAES; GAZIOLA, SALETE APARECIDA; PIOTTO, FERNANDO ANGELO; AZEVEDO, RICARDO ANTUNES. Phytochelatins: Advances in Tomato Research. AGRONOMY-BASEL, v. 15, n. 1, p. 18-pg., 2025-01-01. (22/11018-7, 20/12666-7)

MARQUES, DEYVID NOVAES; STOLZE, SARA CHRISTINA; HARZEN, ANNE; NOGUEIRA, MARINA LIMA; BATAGIN-PIOTTO, KATHERINE DERLENE; PIOTTO, FERNANDO ANGELO; MASON, CHASE; AZEVEDO, RICARDO ANTUNES; NAKAGAMI, HIROFUMI. omparative phosphoproteomic analysis of tomato genotypes with contrasting cadmium toleranc. Plant Cell Reports, v. 40, n. 10, AUG 2021. (16/05788-3, 20/12666-7, 18/20706-9, 16/14349-3, 17/05544-0)