EXPRESSION CONTROL OF IMMUNO-INFLAMMATORY MARKERS IN CONVENTIONAL GLASS IONOMER CEMENT INCORPORATED FROM TITANIUM NANOTUBES ON ODONTOBLASTIC CELLS

• Grant number: 20/15865-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2021
• End date: July 31, 2022
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Kamila Rosamilia Kantovitz
• Grantee: Maria Davoli Meyer
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Campinas , SP, Brazil

Abstract

Titanium dioxide nanotubes (n-TiO2) has been suggested as a promising material for different applications. In order to determine the impact of the addition of n-TiO2 on the physico-chemical properties of glass ionomer cement (GIC), our research group performed a series of in vitro studies (ARP FAPESP #2016/13786-0; 2019/14078-8). In general, the results show that the presence of n-TiO2 into GIC composition produces an improvement in the material's wear resistance and greater hardness without altering the morphology of fibroblasts cultured on the GIC prepared with different concentrations of nanotubes. Despite the significant advance in this area, it is not known if the presence of TiO2 nanotubes in the GIC composition can modulate the expression pattern of inflammatory cytokines. So, the present study aims to determine if the presence of n-TiO2 in GIC composition modifies the expression pattern of inflammatory cytokines in culture of fibroblasts (NIH / 3T3). Different concentrations of n-TiO2 (3%, 5%, 7% in weight) synthesized by the alkaline method (E20 nm) will be incorporated into the GIC [Ketac Molar EasyMix - (KM)]. Odontoblasts will be cultured on GIC discs with and without nanotubes for 18 and 30 h and the following tests will be performed: 1. Cell proliferation assay (Tripan Blue and MTT), 2. Confocal Microscopy (Confocal), 3. Determination of cytokine levels (IL-1², IL-6, IL-10, VEGEF and TNF) using multiplex technology (n=6; 12 and 18 h), 4. Transcript levels for biomineralization markers (DMP1 and DSPP) (n=6, 1, 3 and 5 days). After the results are collected, they will be submitted to statistical analysis, considering the 5% level of significance.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

MEYER, MARIA DAVOLI; COELHO, ROGERIO MENESES IBIAPINA; RANGEL-COELHO, JOAO PEDRO; COSTA, BRUNA CAROLINA; TEIXEIRA, LUCAS NOVAES; MARTINEZ, ELIZABETH FERREIRA; CASARIN, RENATO CORREA VIANA; SANTAMARIA, MAURO PEDRINE; FRANCA, FABIANA MANTOVANI GOMES; NOCITI-JR, FRANCISCO HUMBERTO; et al. Titanium dioxide nanotubes incorporated into conventional glass ionomer cement alter the biological behavior of pre-odontoblastic cells. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 246, p. 10-pg., 2025-02-01. (20/15865-0, 19/14078-8)