Evaluation of the solubility and volumetric change of experimental calcium silicate-based root canal sealers with chitosan biopolymer

• Grant number: 21/08232-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2021
• End date: November 30, 2022
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Marina Angélica Marciano da Silva
• Grantee: Heitor Murillo Carnio
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Chitosan is a natural biopolymer extracted from the arthropod exoskeleton and is a material with wide application due to its antimicrobial, healing, repairing, and bioactive properties, which are interesting for a root canal sealer. In these sealers, solubility is an important property that can influence microbial leakage and filling integrity. Thereby, the purpose of this study is to evaluate the solubility and volumetric change in the use of chitosan biopolymer as a vehicle of an experimental calcium silicate-based root canal sealer and to compare it with another experimental sealer, without the chitosan and with the calcium silicate-based sealer, EndoSequence BC Sealer. The sealers solubility will be based on the ISO 6876/2012 test and Carvalho Junior et al., 2007, (n=6). The volumetric change will be evaluated through the sample volume loss (n =6) of the sealers scanned in micro-CT, before and after 30 days immersion in distilled water. For statistical analysis, the normality and homogeneity data will be evaluated using, respectively, the Shapiro-Wilk and Levene tests. If the data is normal, the two-way ANOVA test will be used to the evaluated statistical difference between groups, and post-hoc with Bonferroni correction will be used to evaluated differences between groups, considered at the significance level less than 5% (P <0.05). (AU)