Isolation and identification of hidrossoluble bioactive peptaibols from produced by the fungus Hypodrea sp.

• Grant number: 21/07343-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2022
• End date: March 31, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Roberto Gomes de Souza Berlinck
• Grantee: Matheus Gotha
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 19/17721-9 - The role of Chemistry in holobiont adaptation, AP.TEM

Abstract

This project aims the isolation non-ribosomal bioactive peptides named peptaibols, produced by the fungus Hypocrea sp. Peptaibols are linear peptides, generally lipophilic, with 4 to 21 amino acids, which have in their structure uncommon amino acids such as ±-aminoisobutyric acid (Aib), and isovaleric acid (Iva) and hydroxyproline imino acid (Hyp). The fungus Hypocrea sp., originating from Antarctica, was shown to be a producer of a series of peptaibols. This project aims at the isolation of water-soluble peptaibols, present in bioactive fractions. Then, to identify the isolated peptaibols using HRMS, MS/MS, and 1D and -2 NMR techniques, as well as to evaluate their cytotoxic, anti-malarial, and antibiotic activity.(AU)