In vitro and in vivo models for assessing the absorption and internalization profile of magnetic nanoparticles for potential biomedical application

• Grant number: 21/06405-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2022
• End date: July 19, 2023
• Field of knowledge: Biological Sciences - Biophysics - Biophysics of Processes and Systems
• Principal Investigator: José Ricardo de Arruda Miranda
• Grantee: Guilherme Augusto Soares
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Magnetic nanoparticles (MNPs) are materials with great potential for applications in healthcare. In this sense, a series of works using the AC Biosusceptometry (BAC) system as an alternative method for detecting and monitoring MNPs were developed. In this project, two lines of investigation are proposed: the absorption of orally administered MNPs, which remains a challenge when dealing with nanostructured systems, and monitoring through magnetic images of the epithelialization process of lesions after keratinocyte grafting. The investigation of the two lines is similar to the previous execution of in vitro studies for later in vivo evaluation. The first line of investigation will be carried out by studying the in vitro absorption of MNPs in Caco-2 cells, a lineage present in the human gastrointestinal tract (GIT) that allows investigating the mechanisms of drugs through the epithelium in different ways. The second investigation will be performed to obtain an equivalent in vitro model of the epidermis by using the cell culture of keratinocytes labeled with MNPs and biological matrices, known as scaffolds. After the in vitro steps, experimental measurements will be carried out in vivo, in which rodents will receive doses of Magnetic Cationic Liposomes (LCMs) (encapsulated with MNPs) to assess absorption via TGI. Regarding another line of study, injured animals will receive grafts from scaffolds developed with keratinocytes labeled with the LCMs. The project has a multicentric execution profile since it has professors from other institutions and even the private sector. It is expected that this work can meet a growing demand for the insertion of nanotechnology directly applied to the area of oral and topical drug delivery; and even in biomedical engineering.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOARES, GUILHERME A.; PEREIRA, GABRIELE M.; ROMUALDO, GUILHERME R.; BIASOTTI, GABRIEL G. A.; STOPPA, ERICK G.; BAKUZIS, ANDRIS F.; BAFFA, OSWALDO; BARBISAN, LUIS F.; MIRANDA, JOSE R. A.. Biodistribution Profile of Magnetic Nanoparticles in Cirrhosis-Associated Hepatocarcinogenesis in Rats by AC Biosusceptometry. PHARMACEUTICS, v. 14, n. 9, p. 19-pg., 2022-09-01. (21/06405-9, 19/11277-0, 21/09829-4, 13/07699-0)