AC biosusceptometry for assessment of renal function and biodistribution of magnetic nanoparticles

• Grant number: 16/23717-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): February 01, 2017
• Effective date (End): December 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Physics - Elementary Particle Physics and Fields
• Principal Investigator: José Ricardo de Arruda Miranda
• Grantee: Milena Foltran de Miranda
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Magnetic nanoparticles (MNPs) are materials with great potential for therapy and diagnosis. From the use of these materials in vivo, there is the need for pre-clinical studies in order to elucidate their behavior in the body, and pharmacokinetics of the parameters of interest. Recent studies have pointed to the use of MNPs providing high rate of renal excretion furthermore, MNPs associated with MRI techniques have been applied to diagnose renal diseases. Despite the MNPs are very studied, their detection in vivo, through its magnetic characteristics, remains restricted to a small number of technical. Thus, the AC Biosusceptometry (BAC), a technique that works as a detector of magnetic materials in biological systems, appears as a versatile and inexpensive alternative for the detection of MNPs in vivo and in real time. The purpose of this work is unprecedented and is the application of the BAC system to detect the purification of blood MNPs coated citrate and different sizes (7:15 nm), as well as your arrival and transit through the kidneys and their levels excretion in the urine, in real time, in healthy animals and animals with induced renal injury. In this way you can assess renal perfusion, biodistribution, transit, accumulation and renal excretion rates of different MNPs, in the kidneys of animals in normal and altered situations, offering new applications of nanostructured materials in angiology, nephrology, biomedical instrumentation, pharmacology, physiology and medical clinic. (AU)