The role of extracellular vesicles of neural precursors in the PDX cells sensitivity to temozolomide

• Grant number: 22/08106-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2022
• End date: April 30, 2024
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Marilene Hohmuth Lopes
• Grantee: João Pedro Alves de Araújo
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/15557-4 - Prion protein and its partners: emerging targets for glioblastoma stem cell based-therapy, AP.JP2
• Associated scholarship(s): 23/06506-5 - Impact of cellular prion protein expression in glioblastoma stem-like cells biology: exploring the biogenesis and cargo of extracellular vesicles, BE.EP.MS

Abstract

Patient-derived xenografts (PDX) are a relevant approach to the study of glioblastomas (GBM) since these cells mimic tumor characteristics in vitro. GBM is the most aggressive and difficult-to-treat primary brain tumor, and its high malignancy and resistance to therapy are due, among other factors, to a subpopulation of tumor stem cells known as glioblastoma stem-like cells (GSCs). Although its origin is still unknown, it is believed that there is a strong association withneural stem cells (NSCs) transformation. Further, it is known that modulation in prion protein (PrPC) expression levels interferes in the biology of GSCs and that the interaction between GBM and NSCs is mostly mediated by extracellular vesicles (EVs), strongly contributing to increased tumor aggressiveness. Given this, GSCs cultivated as neurospheres from PDX cells expressing different levels of PrPC will be initially exposed to EVs from NSCs and then submitted to treatment with temozolomide. Through the evaluation of cell viability, we will know if PDX cells previously treated with EVs from NSCs become more sensitive to chemotherapy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVES DE ARAUJO, JOAO PEDRO; CRUZ, LILIAN; DA SILVA SOUZA, MARIA CLARA; PRADO, MARIANA BRANDAO; ALVES, RODRIGO NUNES; COELHO, BARBARA PARANHOS; SOARES, SAMUEL RIBEIRO; FERNANDES, CAMILA FELIX; LOPES, MARILENE HOHMUTH. Extracellular vesicle derived from neural stem cells as potential vehicles of genetic information to modulate glioblastoma biology. Cancer Research, v. 83, n. 7, p. 2-pg., 2023-04-01. (22/08106-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ARAÚJO, João Pedro Alves de. Effects of neural precursors-derived extracellular vesicles on glioblastoma stem-like cells biology: enlightening the role of cellular prion protein in brain tumor cells interactions.. 2024. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.