Changes in the Eucalyptus leaf phosphoproteome induced by CO2

• Grant number: 22/08845-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): August 01, 2022
• Effective date (End): April 30, 2024
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: Tiago Santana Balbuena
• Grantee: Marina de Lima Nogueira
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated research grant: 18/15035-8 - Probing Eucalytpus plant performance to atmospheric carbon dioxide levels: the source/sink relationships unveiled by targeted proteomics approach, AP.JP2
• Associated scholarship(s): 22/16178-2 - Changes in the Eucalyptus leaf phosphoproteome induced by CO2, BE.EP.PD

Abstract

Protein phosphorylation is one the most important post-translational modifications in plants. Monitoring the phosphorylation status of the leaf proteins may contribute to a better understanding of the regulation processes that occur during photosynthesis and carbon assimilation. The goal of this project is to describe the phosphoproteomics of the Eucalyptus leaves and to identify the phosphorylation changes induced by CO2. For that, we plan to optimize mass spectrometry acquisitions in order to establish a method for parallel (multiple) site assignment and sequence identification. Then, global phosphoproteomics will be carried out by a combination of different enrichment strategies followed by MS data acquisition using the previously established analytical method. Phosphorylation stoichemetric of proteins will be estimated after plant cultivation under controlled conditions.