Protein phosphorylation is one the most important post-translational modifications in plants. Monitoring the phosphorylation status of the leaf proteins may contribute to a better understanding of the regulation processes that occur during photosynthesis and carbon assimilation. The goal of this project is to describe the phosphoproteomics of the Eucalyptus leaves and to identify the phosphorylation changes induced by CO2. For that, we plan to optimize mass spectrometry acquisitions in order to establish a method for parallel (multiple) site assignment and sequence identification. Then, global phosphoproteomics will be carried out by a combination of different enrichment strategies followed by MS data acquisition using the previously established analytical method. Phosphorylation stoichemetric of proteins will be estimated after plant cultivation under controlled conditions.
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