Manufacture of 3D marine sponge from biosilica scaffolds for use in bone tissue engineering and their evaluation of biological effects in vitro

• Grant number: 22/04433-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: December 01, 2022
• End date: January 31, 2025
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Ana Claudia Muniz Renno
• Grantee: Giovanna do Espirito Santo
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Associated research grant: 19/10228-5 - Interdisciplinar net for the development of biotechnological products from marine origin for use in human health: pathological complication of the osteoarticular system, AP.TEM
• Associated scholarship(s): 23/10900-0 - 3D coating of nanobiosilica nanobiosilica coating extracted from marine sponges to improve osseointegration of titanium implants., BE.EP.MS

Abstract

In recent years, the increasing occurrence of bone fractures in their critical state has reached approximately 200 million people around the world, resulting in high costs for the proper treatment. Thus, the use of bioceramics is an affordable and viable alternative for this purpose, among which it is possible to mention the use of biosilica extracted from marine sponges, given that it has proven osteogenic effects in in vitro studies and a lower cost of implantation by through bone substitutes. In order to optimize the manufacture of scaffolds, the 3D printing technique has made it possible to singularize their three-dimensional structures, providing cell adhesion and proliferation. Once the problem has been raised and potential resources highlighted, it is hypothesized that marine biosilica scaffolds manufactured by 3D printing simultaneously exhibit efficient properties for cellular stimulation. Therefore, it aims to compare two manufacturing models of 3D marine biosilica scaffolds and evaluate their physicochemical characteristics and their biological effectiveness in in vitro assays. For the physicochemical analysis, pH and mass loss tests will be carried out. Then, Scanning Electron Microscopy (SEM), Fourier Infrared Spectroscopy (FTIR) and Energy Dispersive Spectroscopy (EDS), to analyze their chemical composition and the surface of the scaffolds. In addition, in vitro assays will be performed with the MC3T3 (osteoblasts), L929 and HFF-1 (murine and human fibroblasts, respectively) strains to evaluate cell adhesion and proliferation, and later cell viability assay using MTT. For the evaluation of the results by statistical analysis, the ANOVA-Twoways analysis of variance will be adopted, as well as the POST-HOC Turkey test, with a significant difference (p<0.05).