Volumetric analysis of substance loss of rat's teeth submitted or not to erosive challenges and to previous treatment with TiF4 varnish

• Grant number: 22/13803-3
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: December 15, 2022
• End date: February 14, 2023
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Ana Carolina Magalhães
• Grantee: Júlia França da Silva
• Supervisor: Marcella Esteves Oliveira
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Institution abroad: University of Bern, Switzerland
• Associated to the scholarship: 21/06143-4 - Abrasive potential of dentifrices containing activated charcoal on dentin eroded: in vitro study, BP.IC

Abstract

Although previous in vitro and in situ studies have shown that TiF4 varnish (single application) can reduce tooth enamel erosion, no in vivo studies have been performed to confirm their protective role. Thus, the objectives of this study were to develop an animal model that can facilitate understanding the development of erosive tooth wear and to evaluate the role of TiF4 on the prevention of erosive tooth wear. For this, forty-eight male Wistar rats will be divided into three groups: 1 - experimental TiF4 varnish (2.45% F-, pH 1); 2 - NaF varnish (2.45% F-, pH 5); 3 - placebo varnish (pH 5). Of the 16 mice per group, 8 will be subjected to erosive challenges and the other 8 mice will receive water instead of an erosive agent. The varnishes will be applied at once. The rats from the "erosion" subgroup will receive a degassed beverage (Sprite®), while the "control" rats will receive tap water, both ad libitum. After an experimental period of 28 days, the rats will be sacrificed and the mandibles will be dissected for clinical evaluation. For the quantitative analysis of tooth substance loss and topographical characterization occurring at the rat molars tooth surfaces (from 1st to 3rd molars) a 3D confocal laser microscope will be used (VK- X3000, Keyence, Neu-Inseburg, Germany). Subsequently all images of each tooth will be analyzed with a compatible software including assessment of volume of grooves, height and surface losses. The data will be submitted to appropriate statistical analysis (two-way ANOVA, p<0.05). (AU)