Formulations of experimental dentifrices containing fluoride, stannous and different polymers for the prevention of erosive tooth wear on enamel and dentin.

• Grant number: 21/11100-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2022
• End date: July 08, 2026
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Taís Scaramucci Forlin
• Grantee: Raissa Manoel Garcia
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 23/02049-9 - Effect of experimental toothpastes containing fluoride, tin and different polymers against enamel demineralization, BE.EP.DR

Abstract

The present study has two objectives: 1. To formulate experimental dentifrices containing different polymers (CHI: chitosan; LPP: sodium linear polyphosphate; MPC: 2-methacryloyloxyethyl phosphorylcholine and Carbopol) associated to fluoride (F-, as NaF) and to fluoride plus stannous (Sn2+, as SnCl2), which will be characterized in terms of pH and fluoride release; 2. To test the efficacy of these formulations in the prevention of erosive tooth wear on enamel and dentin. For this, fragments of each substrate will be used (4 mm × 4 mm × 2 mm), and they will be obtained from bovine incisors. The fragments will be ground flat, polished and randomly distributed into the following experimental groups (n = 10 / substrate): 1. Negative control - brushing with artificial saliva; 2. Positive control - conventional fluoride dentifrice (Elmex Erosion, 1400 ppm F-); 3. Experimental placebo dentifrice (without actives); 4. Experimental dentifrice with CHI; 5. Experimental dentifrice with CHI + F; 6. Experimental dentifrice with CHI +F+Sn; 7. Experimental dentifrice with LPP; 8. Experimental dentifrice with LPP + F; 9. Experimental dentifrice with LPP + F + Sn; 10. Experimental dentifrice with MPC; 11. Experimental dentifrice with MPC + F; 12.Experimental dentifrice with MPC + F +Sn; 13. Experimental dentifrice with Carbopol; 14. Experimental dentifrice with Carbopol + F ; 15. Experimental dentifrice with Carbopol + F + Sn. Then, the specimens will be subject to an erosion-abrasion cycle, consisting of 5 min of immersion in 1% citric acid solution, followed by immersion in artificial saliva for 60 min, 4 times a day. Brushing will be performed in a brushing machine, using soft brushes and a slurry of toothpaste and artificial saliva, at a ratio of 1: 3, twice a day. This procedure will be repeated for 5 days. After cycling, specimens will be evaluated for surface loss (in ¼m) using an optical profilometer. The dentifrices will be characterized in relation to their pH and potentially available fluoride (analyzed in the dentifrice suspensions). Data obtained will be statistically evaluated, considering a significance level of 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARCIA, RAISSA MANOEL; RIGO, LETICIA MARIA BALBINO; LIMA, LEONARDO CUSTODIO; SAKAE, LETICIA OBA; CAVALLI, VANESSA; BORGES, ALESSANDRA BUHLER; SCARAMUCCI, TAIS. Polymer-Based Toothpastes and Their Ability in Control Tooth Extrinsic Stain. Journal of Esthetic and Restorative Dentistry, v. N/A, p. 8-pg., 2024-12-27. (21/11100-2)