In vitro bioavailability of main mycotoxins found in food samples and exposure assessment

• Grant number: 22/15367-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: February 01, 2023
• End date: December 27, 2025
• Field of knowledge: Agronomical Sciences - Food Science and Technology
• Principal Investigator: Carlos Augusto Fernandes de Oliveira
• Grantee: Esther Lima de Paiva
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated research grant: 19/21603-1 - Biomarker approaches for assessing the early life exposure to multiple mycotoxins in the diet, AP.TEM
• Associated scholarship(s): 24/01212-6 - Combined effects of mycotoxins and metals present in infant foods : analysis in different target organs, BE.EP.PD

Abstract

This Project aims to determine the "in vitro" bioavailability of regulated mycotoxins in Brazil, and adjust the exposure assessment of children to mycotoxins obtained in another Work Package of the Thematic Project (WP-3) through food data and biomarkers present in the urine. A set of "in vitro" methodologies using a digestion method that simulates the digestive process, and a caco-cell system that simulates the transport across the intestinal membrane, from apical to basolateral sides of intestinal membrane, will allow to elucidate the bioavailable portion that arrives to the intestinal and that is able to cause toxic effects on cells. Cell viability will be assessed using the 3-{4,5-dimethylthiazol-2-yl}diphenyltetrazolium bromide (MTT) assay. The experiments will be performed in triplicate, with the presence or absence of three types of standard meals (commercial available infant formulae, cereal-based food for babies, and fruit-based product) previously spiked with AFB1, FB1, OTA, ZEN and DON alone or in combination, besides controls (not spiked). The spiking levels of each mycotoxin will be defined based on the analytical results obtained in WP-3 for the aforementioned types of infant foods, considering the minimum and the maximum concentrations of each mycotoxin found in the products. The bioavailability data generated in the experiments will be used to accurate the estimated exposure determined in WP-3 of the Thematic Project. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALI, SHER; REZENDE, VANESSA THEODORO; ULLAH, SANA; DE PAIVA, ESTHER LIMA; TONIN, FERNANDO GUSTAVO; ABDULLAH; CORASSIN, CARLOS HUMBERTO; DE OLIVEIRA, CARLOS AUGUSTO FERNANDES. Food processing and challenges in the food production and quality: The foodomics approach. FOOD BIOSCIENCE, v. 56, p. 13-pg., 2023-10-09. (22/03952-1, 19/21603-1, 22/15367-6, 23/05989-2)

PIRES, ROGERIO CURY; CALUMBY, JULIA DA COSTA; ROSIM, ROICE ELIANA; PIRES, ROGERIO D'ANTONIO; BOROWSKY, ALINE MOREIRA; ALI, SHER; DE PAIVA, ESTHER LIMA; SILVA, RAMON; PIMENTEL, TATIANA COLOMBO; DA CRUZ, ADRIANO GOMES; et al. Evaluation of Ability of Inactivated Biomasses of Lacticaseibacillus rhamnosus and Saccharomyces cerevisiae to Adsorb Aflatoxin B₁ In Vitro. FOODS, v. 13, n. 20, p. 12-pg., 2024-10-01. (22/03952-1, 19/21603-1, 22/15367-6, 22/07439-7)

DE PAIVA, ESTHER LIMA; ALI, SHER; VASCO, ELSA REIS; ALVITO, PAULA CRISTINA; OLIVEIRA, CARLOS AUGUSTO FERNANDES. Bioaccessibility data of potentially toxic elements in complementary foods for infants: A review. Food Research International, v. 174, p. 11-pg., 2023-10-17. (22/03952-1, 22/15367-6)