Changes in the Eucalyptus leaf phosphoproteome induced by CO2

• Grant number: 22/16178-2
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: April 30, 2023
• End date: July 29, 2023
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: Tiago Santana Balbuena
• Grantee: Marina de Lima Nogueira
• Supervisor: Ive de Smet
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Institution abroad: Vlaams Instituut voor Biotechnologie (VIB), Belgium
• Associated to the scholarship: 22/08845-9 - Changes in the Eucalyptus leaf phosphoproteome induced by CO2, BP.PD

Abstract

Protein phosphorylation is one the most important post-translational modifications in plants and a key event in the regulation of protein activity. The reversible phosphorylation/dephosphorylation steps can modulate signaling processes, control enzymatic activity, modify sub-cellular structures and regulate gene expression. Monitoring the phosphorylation status of the leaf proteins from plants grown in different CO2 concentrations may contribute to a better understanding of the post-translational processes that occur during photosynthesis and carbon assimilation. The goal of this project is to acquire and process phosphoproteomics data using a well established computational pipeline developed by the De Smet group at the VIB-UGent-Institute for Plant Systems Biology. Global leaf phosphoproteome analysis will be carried out using a combination of different enrichment strategies followed by MS data acquisition according to a previously established analytical method. The stoichiometry of protein phosphorylation will be estimated after plant cultivation under controlled conditions under a dedicated pipeline. Apart from the scientific results, the research mission will assist in the implementation of a phosphoproteome sample processing strategy and a data analysis workflow that may be used in any propospective research project from the group and/or from collaborators. (AU)