Metabolite annotation and identification in wild and modified cell strains using untargeted and integrated NMR approach

• Grant number: 23/02338-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: April 01, 2023
• Status: Discontinued
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Ljubica Tasic
• Grantee: Thyerre Santana da Costa
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 20/08615-8 - Protein exosites, cryptic sites and moonlighting: identification, functional mapping and effects of changes in structure, AP.TEM
• Associated scholarship(s): 24/13937-5 - METABOLITE ANNOTATION AND IDENTIFICATION IN WILD AND MODIFIED CELL STRAINS USING UNTARGETED AND INTEGRATED NMR APPROACH, BE.EP.DD

Abstract

Metabolomics is an "omics" platform applied in chemical and biochemical studies of organisms. From the initial need to understand the genetic information stored in cells (genomics), the focus is shifted to the product encoded by genes, that is, structures and functions of proteins (proteomics), to the study of the end products of the luxuries between the genome, proteome with the environment, that is, metabolomics. Thus, this technique investigates the metabolic state of organisms, correlating it with a wide and complex ingestion network to define homeostasis and pathophysiology. Compared to other "omics" techniques, metabolomics has the advantage of being a relatively fast, inexpensive and sensitive technique for detecting subtle differences between multiple samples. Data on the possible ways in which specific proteins can participate, within these physiological environments teeming with biomolecules, are fundamental to understanding the roles that are often played by proteins. It is essential to use a fast and reproducible instrument that requires a simple and fast experimental technique. Analysis of 1H-NMR spectra meets this requirement in addition to allowing simultaneous monitoring, without phase separation, of a variety of compounds and is non-destructive. Nuclear Magnetic Resonance (NMR) and chemometric analysis have been widely used in metabolic studies of biofluids or tissues and represent a main technique for this research. The core of this proposal is centered on biological peptide proteins, which are currently being studied by the research groups that are part of the thematic project, to understand their proteins with peptides and with other proteins to discern their possible multiple roles. The team involved in this proposal is formed by a researcher who has already established collaborations for years, which yielded several scientific articles and patents. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, FABIO HENRIQUE DOS SANTOS; DELGADO, GONZALO GARCIA; COSTA, THYERRE SANTANA DA; TASIC, LJUBICA. Applications of fluorescence spectroscopy in protein conformational changes and intermolecular contacts. BBA ADVANCES, v. 3, p. 13-pg., 2023-01-01. (23/02338-0, 14/50867-3, 20/08615-8)

DA COSTA, THYERRE SANTANA; DA SILVA, MARIANA RODRIGUES; BARBOSA, JULIO CESAR JERONIMO; DAS NEVES, UEDSON DA SILVA; DE JESUS, MARCELO BISPO; TASIC, LJUBICA. Biogenic silver nanoparticles' antibacterial activity and cytotoxicity on human hepatocarcinoma cells (Huh-7). RSC ADVANCES, v. 14, n. 4, p. 13-pg., 2024-01-10. (23/02338-0, 23/06874-4, 22/07854-4, 20/08615-8, 20/01218-3)