Bond strength and adhesive interface quality of a new premixed bioceramic endodontic sealer

• Grant number: 23/04148-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2023
• End date: April 30, 2024
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Fabiane Carneiro Lopes Olhê
• Grantee: Gustavo Creazzo
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The aim of the present study will be to evaluate the bond strength (BS) and the quality of the adhesive interface of the premixed bioceramic endodontic sealers AH Plus Bioceramic (Dentsply), Bio-C Sealer (Angelus) and NeoSEALER Flo (Avalon Biomed). Thirty-six single-rooted human teeth with circular canals will have their crowns removed and the root portion subjected to biomechanical preparation with the Reciproc system (R50) and irrigation with 2.5% sodium hypochlorite. After a final irrigation protocol with 17% EDTA for 5 minutes, followed by a final rinse with 2.5% sodium hypochlorite, the specimens will be randomly distributed into 3 experimental groups according to the endodontic sealer used (n=12): AH Plus Bioceramic, Bio-C Sealer and NeoSEALER Flo. The root canals will be dried and filled using the single cone technique (R50). In 2 samples of each group, the sealer will have calcein added during manipulation. After 3 times the sealer setting time, the specimens will be cross-sectioned into slices of 1 mm (± 0.1 mm) thick, obtaining 3 slices from each root third. The first and second slices of each third will be used for the push-out test followed by the analysis of the failure pattern, while the third slice of each root third will be used for the analysis of the quality of the adhesive interface by confocal laser microscopy (CLM). In the samples in which the sealer receives dye, 2 slices of each third will be prepared for analysis of the intratubular penetration of the sealer by means of confocal laser fluorescence microscopy (CLFM). The BS data, adaptation of the adhesive interface and intratubular penetration of the sealer will be submitted to statistical analysis.