EVALUATION OF THE ANTIMICROBIAL RESISTANCE PROFILE OF BACTERIAS ISOLATED FROM ULCERATIVE KERATITIS IN DOGS.

• Grant number: 23/07736-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2023
• End date: August 31, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Paola Castro Moraes
• Grantee: Mayara Paula Paglione
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Ulcerative keratitis is commonly found in veterinary routine, with higher incidence in dogs belonging to brachycephalic breeds. They have a multifactorial etiology and can lead to a decline in visual quality or total loss of vision, especially in cases where treatment is neglected. The affection usually presents contamination due to the presence of commensal bacteria of the ocular microbiota, thus, the treatment essentially must contain antimicrobials that are effective so that there is no progression of the picture. However, it has been increasingly common in veterinary ophthalmology challenges for facing complex cases, with bacteria resistant to available antimicrobials, thus conferring a unique health risk. It is, therefore, necessary that therapeutic management be guided by antimicrobial susceptibility testing, both for the success of treatment and for monitoring the pattern of resistance and consequently establishing measures for the prevention and containment of bacteria resistant. Based on this, the objective is to evaluate the resistance profile of bacteria to the available active ingredients, in order to understand the dynamics of the bacterial population present in the cities of Jaboticabal-SP and Araçatuba-SP. To this end, the samples collected in a previous study will be submitted to antimicrobial susceptibility testing by disc diffusion and the isolates of Staphylococcus spp. will be submitted to PCR to identify penicillin and beta-lactam resistance genes.