Development of lipase purification strategies by physical adsorption on organic supports as a low-cost alternative

• Grant number: 24/13073-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2024
• Status: Discontinued
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Heitor Buzetti Simões Bento
• Grantee: Milena Lorenzi da Silva
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 25/02380-2 - Evaluation of the potential of protein immobilization by physical adsorption on carbon nanotubes and xerogels as a low-cost purification tool., BE.EP.IC

Abstract

Lipases are valuable enzymes for the food, cosmetics, pharmaceutical and environmental control industrial sectors, where their applications include the catalysis of hydrolysis, esterification, acidolysis, aminolysis, among other reactions. Currently, such proteins are mostly obtained from microbiological sources, whose production requires the use of different downstream processes for the separation and purification of the biocatalyst, such as filtration, centrifugation, precipitation and chromatography. Such steps impact not only the production rate bur mainly the cost of the global process, representing up to 80% of the commercial value of these proteins. Therefore, it is understood that the development of simple and low-cost techniques for the separation and purification of lipases brings together academic and industrial relevance. Therefore, this project proposes the study of the use of the enzyme immobilization by physical adsorption technique , a simple and low-cost method, on different supports, as a low-cost step for the purification of lipases of microbial origin.