BOND STRENGTH OF BIS-GMA-FREE UNIVERSAL ADHESIVE SYSTEMS IN ERODED DENTIN

• Grant number: 24/15569-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2025
• End date: December 31, 2025
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Roberta Tarkany Basting Höfling
• Grantee: Ana Clara Cassinelli Francisco
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil

Abstract

Universal adhesive systems are materials used to ensure micromechanical adhesion between the interface of the dentin substrate and resin materials, which have Bis-GMA (bisphenol glycidyl dimethacrylate) as the main component of the organic matrix. Despite this, its use has been questioned, due to its potential systemic effects, as it is a derivative of BPA. On the other hand, dentin that presents itself as an avascular mineralized tissue may present pathological processes, such as dental erosion, which will cause a softening of the surface causing exposure of a less mineralized dentin. These processes will lead to changes in its composition, and may hinder the union of universal adhesive systems to the dentin itself, which is now characterized as eroded dentin. Therefore, the proposed study will aim to analyze the bond strength and nanoleakage of three Bis-GMA-free universal adhesive systems (Single Bond Universal/ Scotchbond Universal Plus/ Amber APS Universal Plus) in eroded dentin (specimens immersed in orange juice - acidic drink) and in healthy dentin (specimens immersed in artificial saliva). 60 human third molars without cracks or any alteration in enamel and/or dentin will be selected and standardized for the in vitro study. The teeth will be divided into three groups, respecting the application of the different types of adhesive systems (following the manufacturer's guidelines) and for the restorations, composite resin (Filtek Z350 XT, 3M ESPE, St. Paul, Minnesota, USA) will be used, applied by the incremental and photoactivated technique (Valo Corded, Ultradent, Salt Lake City, UT, USA). The bond strength by microtraction will be measured, the failure mode will be evaluated, in addition to this the bond interface will be examined by scanning electron microscopy and nano leakage evaluations. The analyses will be made considering the significance level of 5%.