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Impact of drying protocol on bond strength and adhesive interface quality of premixed and powder/liquid bioceramic filling cement

Grant number: 24/18887-6
Support Opportunities:Scholarships in Brazil - Master
Start date: August 01, 2025
End date: July 31, 2026
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Fabiane Carneiro Lopes Olhê
Grantee:Gustavo Creazzo
Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The aim of this study is to evaluate the bond strength (BS) and the quality of the adhesive interface using different root canal drying protocols for the bioceramic sealer BioRoot RCS (Septodont) in powder-liquid form and the pre-mixed BioRoot Flow (Septodont). Sixty single-rooted human teeth with a single, circular canal will have their crowns removed, and the root portion will undergo biomechanical preparation using the Reciproc system (R50) with irrigation using 6 mL of 2.5% sodium hypochlorite (NaOCl). After a final irrigation protocol with 6 mL of 17% EDTA for 5 minutes, followed by rinsing with 6 mL of distilled water, the specimens will be randomly distributed into three experimental groups according to the drying protocol and into two subgroups based on the bioceramic sealer used. Dry: Complete drying with a White Mac tip (Ultradent, Indaiatuba, SP, Brazil) for 5 seconds, followed by aspiration using purple and green capillary tips for 5 seconds and paper points.Slightly moist: Drying with the White Mac tip (Ultradent, Indaiatuba, SP, Brazil) and the purple and green capillary tips, without the use of paper points to maintain residual moisture. Moist: Drying with the White Mac tip followed by a single paper point. The samples will be obturated using the single-cone technique (R50) with BioRoot RCS or BioRoot Flow (n=10) and stored for 30 days. The specimens will then be transversely sectioned into 1 mm (± 0.1 mm) thick slices, obtaining three slices from each root third. The first and second slices of each third will be used for the push-out test followed by failure pattern analysis, while the third slice of each third will be used to analyze the quality of the adhesive interface through confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), followed by an analysis of the chemical composition of the adhesive interface using Raman spectroscopy. Bond strength and adhesive interface adaptation data will be subjected to statistical analysis. (AU)

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