NGAL as a biomarker of acute kidney injury in rats undergoing balanced anesthesia - influence of fluid resuscitation with Ringer Lactate or hydroxyethyl starch: comparative assessment of the Rifle criteria

• Grant number: 10/12862-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2010
• End date: October 31, 2011
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Norma Sueli Pinheiro Módolo
• Grantee: Gustavo Iacomini Ida
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

20 Wistar rats weighing 250-300 grams are anesthetized with isoflurane and remifentanil by continuous infusion to achieve D nephrectomy and renal artery clamping and for a period of 30 minutes. The animals will be divided into two study groups according to volume replacement performed: Ringer's lactate or starch hidroxietil. Blood samples taken before performing nephrectomy and renal ischemia after releasing the clamp, after 30 minutes and postoperative immediately for assay of creatinine and NGAL. These doses will be compared to the criterion of Rifle. (AU)