DNA damage induced by beta-carotene oxidation products

• Grant number: 10/10933-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2010
• End date: June 30, 2012
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal Investigator: Marisa Helena Gennari de Medeiros
• Grantee: Raul Xavier Do Nascimento
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

We have investigated the reactions of beta-carotene and two of its oxidation products, retinal and beta-apo-8'-carotenal, with 2'-deoxyguanosine to evaluate their DNA damaging potential. A known mutagenic adduct, 1,N2-etheno-2'-deoxyguanosine, was isolated and characterized on the basis of its spectroscopic features. After treatment of calf thymus DNA with beta-carotene or beta-carotene oxidation products, significantly increased levels of the etheno adduct were detected and quantified in DNA by a sensitive LC/ESI/MS-MS technique. For comparative purposes, in the present study, levels of 8-oxo-7,8-dihidro-2'-desoxiguanosine will be evaluated in human lung cells (IMR-90) exposed to beta-carotene and its oxidation products.