Impact of the GSTP1 Alw 26I variant as detoxication enzyme of xenobiotics in Parkinson's Disease

• Grant number: 09/17222-0
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): June 01, 2010
• Effective date (End): May 31, 2011
• Field of knowledge: Health Sciences - Medicine
• Principal researcher: Dorotéia Rossi Silva Souza
• Grantee: Gabriela dos Santos Longo
• Home Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

Parkinson´s disease (PD) is characterized by motor alterations resulting from nigrostriatal dopaminergic cells degeneration, which modifies the amount of dopamine in striatum unbalancing the motor circuit responsible for the normal movements. Genetic predisposition, aging and environmental toxins contribute to Parkinson's disease etiopathogenesis. In this context, toxins accumulation related to genetic variants of glutathione-S-transferases (GST) can injury dopaminergic neurons of substantia nigra, leading to PD. This study aims to analyze the frequency of genetic variant GSTP1 Alw 26I in PD patients; evaluate the association between GSTP1 Alw 26I polymorphisms and PD onset; and verify the association of GSTP1 Alw 26I variant and environmental toxins exposure in PD patients. One hundred and fifty patients with PD and 150 individuals without PD diagnosis (controls) will be studied. Allelic variants will be identified by polymerase chain reaction (PCR) and digested by Alw 26I enzyme for restriction fragment length polymorphism (RFLP) analysis, in 6% polyacrylamide gel. The statistical analysis will consist in parametric and nonparametric methods, according to the nature of the involved variables. The adopted significance level will be 0,05. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GREGORIO, MICHELE L.; PINHEL, MARCELA A. S.; SADO, CAROLINE L.; LONGO, GABRIELA S.; OLIVEIRA, FABIO N.; AMORIM, GISELE S.; NAKAZONE, MARCELO A.; FLORIM, GREICIANE M.; MAZETI, CAMILA M.; MARTINS, DENISE P.; TOGNOLA, WALDIR A.; BRANDAO, ANTONIO C.; PINHEIRO JUNIOR, SIDNEY; DE GODOY, MOACIR F.; SOUZA, DOROTEIA R. S. Impact of Genetic Variants of Apolipoprotein E on Lipid Profile in Patients with Parkinson's Disease. BIOMED RESEARCH INTERNATIONAL, 2013. Web of Science Citations: 10.