Tissue response to dentine tubes irrigate with 23 silver nanoparticles or 2.5% sodium hypochlorite

• Grant number: 09/15981-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2009
• End date: November 30, 2010
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: João Eduardo Gomes Filho
• Grantee: Fernanda Fragoso Ferreira de Melo
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The aim of this study was to evaluate the tissue response to implanted dentine tubes irrigated with silver nanoparticles dispersion or 2.5% sodium hyppochlorite. Thirty rats will individually receive two dentine tubes irrigated with 23ppm silver nanoparticles dispersion, 2.5% sodium hypochlorite or saline as controle. The observation periods will be 7 and 30 days. After each period of time, fifteen animals will be killed and the tubes and surrounding tissue removed, fixed and prepared to be analyzed in light microscope with glycol methacrylate embedding, 3 mm serial cutting and HE staining. Scores will be defined as follows: 0, none or few inflammatory cells, no reaction; 1, <25 cells, mild reaction; 2, 25 to 125 cells, moderate reaction; 3, >125 cells, severe reaction. Fibrous capsule was categorized as "thin" when thickness was < 150 ¼m and "thick" at > 150 ¼m. Necrosis and formation of calcification were recorded. The data will be statistically analyzed using Kruskal Wallis test.