Study of the mutagenic activity of azo dyes processing plants effluent - which compounds should to be regulated in the aquatic environment?

• Grant number: 01/14521-5
• Support Opportunities: Scholarships abroad - Research
• Start date: September 01, 2002
• End date: January 31, 2003
• Field of knowledge: Biological Sciences - Genetics - Mutagenesis
• Principal Investigator: Gisela de Aragão Umbuzeiro
• Grantee: Gisela de Aragão Umbuzeiro
• Host Investigator: David Demarini
• Host Institution: Companhia Ambiental do Estado de São Paulo (CETESB). Secretaria de Meio Ambiente, Infraestrutura e Logística (São Paulo - Estado). São Paulo , SP, Brazil
• Institution abroad: United States Environmental Protection Agency (EPA), United States

Abstract

Dyes used in industries that presented high mutagenic effluent in the past will be selected. The process of dyeing will be simulated and the resultant complex mixtures will be tested in the Salmonella microsome/mutagenicity test, the comet assay and the formation of DNA adducts.. Chemical characterization will be performed using HPLC/MS. A pilot study will be conducted. The compounds suspected to be responsible for the mutagenic activity observed in the complex mixture will be tested in the same bioassays. As a consequence, standards will be proposed to adequately regulate mutagenic compounds originated from dyeing plant discharges in the aquatic environment. (AU)