Peripheral insulin-sensitivity and inflammation response under obesogenic diets, fish oil supplementation and absence of TLR-4.

Author(s)

Laureane Nunes Masi

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-08-25

Examining board members

Rui Curi; Eliana Hiromi Akamine; Fabio Bessa Lima; Simão Augusto Lottenberg; Jorge Mancini Filho

• Advisor: Rui Curi

Abstract

The aim of this study was to investigate the association between inflammation and insulin resistance (IR) under obesogenic diets, fish oil supplementation (FOS) and TLR-4-/-. The mice were fed a balanced diet (BD), BD and condensed milk (BD+CM), high-fat diet (HFD) or high-fat diet and condensed milk (HFD+CM). The BD+CM and HFD increased: body weight gain (BWG), F4/80 and leptin in adipose tissue (AT). The HFD+CM compared to HFD increased: HOMA-IR, the contents of IL-6, IL-10, TNF-a, IL-1b, ICAM-1, VCAM-1 and leptin in AT. As compared to BD+CM, HFD+CM increased: BWG, HOMA-IR, F4/80 and reduced the leptin in AT. The FOS+BD reduced F4/80, TNF-a and TLR-4 in the liver. The FOS+HFD reduced BWG, HOMA-IR and increased the adiponectin in AT. TLR-4 deletion increased BWG in the FOS+BD and mesenteric fat in the FOS+HFD groups. The high consumption of fat or sugar induced obesity and the association of both potentiated this effect leading to IR and inflammation in adipose tissue and perivascular fat. FO supplementation attenuated the inflammation when associated with BD and the obesity and IR induced by HFD in a TLR-4 dependent-manner. (AU)

• FAPESP's process: 10/08872-9 - Effects of a high saturated fat diet on inflammatory response in aortic endothelium of TLR-4 (-/-) mice supplemented with omega-3 and omega-6 fatty acids
• Grantee: Laureane Nunes Masi
• Support Opportunities: Scholarships in Brazil - Doctorate