Bone repair evaluation in bone/implant interface in pinealectomized rats: histological, immunohistochemistry, histometric, biomechanics and microCT analysis

Author(s)

Tárik Ocon Braga Polo

• Document type: Master's Dissertation
• Press: Araçatuba. 2016-03-04.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba
• Defense date: 2016-02-04
• Advisor: Roberta Okamoto; Dóris Hissako Sumida

Abstract

This study aimed to investigate the interface bone/implant in pinealectomized rats treated with melatonin. 30 rats, male, adults were divided into 3 groups: control (CO), pinealectomized (PNX) and pinealectomized with melatonin (PNXm). After 30 days of the pinealectomy, one implant was installed on each tibia and euthanasia was performed at 42 days. The samples were submitted to histometric (BIC and NB), biomechanical (reverse torque), microtomography and immunohistochemical analysis. The quantitative data were subjected to statistical analysis (P<0.05). For the histometric results, BIC values to PNX were lower when compared to PNXm (P=0.0393), whereas NB did not showed difference among the groups (P=0.761). Reverse torque did not show difference between the groups PNX and PNXm (P=0,5000), and the interactions CO vs PNXm, and CO vs PNX showed significantly difference (P<0.05). The volumetric parameters BV/TV (P=0.933); Tr.N (P=0.933); Tb.Th (P=0.933), Tb.Sp (P=0.200) did not present statistical difference. The immunolabeling for OPG, RANKL, TRAP and osteocalcin showed similarity among the groups, despite the PNXm it seems to resemble to physiological conditions (CO). Therefore, melatonin improved the bone turnover in peri-implant region in pinealectomized rats. (AU)

• FAPESP's process: 14/11920-6 - Evaluation of bone healing in bone/implant interface in pinealectomized rats: histologicc, histometric, immunohistochemical, biomechanical and microCT analysis
• Grantee: Tárik Ocon Braga Polo
• Support Opportunities: Scholarships in Brazil - Master