Biomimetic nanoreactors to the based peroxidase in MIP: a promising strategy for determination of phenolics compounds

Author(s)

Wilney de Jesus Rodrigues Santos

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Química
• Defense date: 2009-04-08

Examining board members

Lauro Tatsuo Kubota; Lucio Angnes; Hideko Yamanaka; Inez Valéria Pagotto Yoshida; Pedro Luiz Onófrio Volpe

• Advisor: Lauro Tatsuo Kubota

Abstract

The present work describes the applications of biomimetic nanoreactor to the based peroxidase in molecularly imprinted polymers (MIP) as a promising tool for determination of substances of high biological and environmental interest, such as phenolic compounds (4-aminophenol and serotonin). In this sense, the synthesis of MIPs was based on the conventional polymerization in bulk. Each polymer was synthesized from methacrylic acid (functional monomer), ethylene glycol dimethacrylate (cross-linking reagent), 2,2'-azobis-isobutyronitrile (initiator), in the presence of Fe(III)protoporphyrin(IX) (hemin) as a catalytic center, which is responsible for the mimic of the active site of peroxidase, creating therefore, a molecularly imprinted polymer active catalytically for the recognition of the 4-aminophenol and serotonin (template molecules). Furthermore, in order to evaluate the selectivity of the material, were prepared, parallel, polymers without the molecular impression (NIP - Non imprinted polymers) and also in the hemin absence. The MIPs were characterized by the techniques of infrared spectroscopy, specific surface area, specific pore volume, thermogravimetric analysis, scanning electron microscopy. Kinetic parameters, including values for maximum rate, Vmax and Michaelis-Menten apparent constant, Km were obtained from Lineweaver-Burk plots. For analytical application, in samples of water and blood serum, amperometric systems were optimized through multivariate analysis (AU)

• FAPESP's process: 06/00519-2 - Development of biomimetic sensors based on molecular imprinted polymers to determine species of biological and environmental interest
• Grantee: Wilney de Jesus Rodrigues Santos
• Support Opportunities: Scholarships in Brazil - Doctorate