Dispositivos analíticos a base de papel para análise de amostras de interesse clínico e alimentício

Author(s)

Emilia Katarzyna Witkowska Nery

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Química
• Defense date: 2015-10-08

Examining board members

Lauro Tatsuo Kubota; Dosil Pereira de Jesus; Ivo Milton Raimundo Júnior; Lucio Angnes; Eduardo Mathias Richter

• Advisor: Lauro Tatsuo Kubota

Abstract

Paper-based sensors accompany analytical chemistry for centuries in form of spot-tests, litmus paper and lateral flow assays based on nitrocellulose but just in the last few years this humble substrate was rediscovered as a platform for integrated microfluidics. Mechanical properties, three-dimensional fibrous structure, biocompatibility and biodegradability, easiness of production and modification, reasonable price, and availability all over the world are among others the reasons that made paper the first choice substrate for sensors and integrated sensing platforms. The following project is aimed at developing POCT (Point of Care Test) devices based on paper. Such tests can be carried out by an unqualified professional, with the results analyzed immediately or sent to a laboratory. The following thesis will describe stages of development of a platform for analysis of glucose, cholesterol and uric acid in biological samples (colorimetric and electrochemical detection), evaluate methods of quantification of proteins immobilized on paper, and review enzyme immobilization methods. The second part will be dedicated to the development of potentiometric sensors, and their utilization in electronic tongue systems. Those systems will be applied for analysis of beverages: detection of falsified mineral waters, classification of beers and wines. This part includes development of potentiometric sensors of different architectures, integration of reference electrode and minimization of sample volume necessary for the analysis (AU)

• FAPESP's process: 11/10883-1 - Development of new paper based analytical device for simultaneous detection of cholesterol, glucose and uric acid.
• Grantee: Emilia Katarzyna Witkowska Nery
• Support Opportunities: Scholarships in Brazil - Doctorate