Biocatalytic processes applying epoxide hydrolases, oxidoreductases and transaminases

Author(s)

Bruna Zucoloto da Costa

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Química
• Defense date: 2015-02-24

Examining board members

Anita Jocelyne Marsaioli; Sonia Rodríguez Giordano; Leandro Helgueira de Andrade; Luciana Gonzaga de Oliveira; Cátia Ornelas

• Advisor: Anita Jocelyne Marsaioli

Abstract

Biocatalytic processes have been addressed in this thesis from the microorganism screening for the selection of suitable biocatalysts to the use of isolated enzymes in biotechnological reactions. The first chapter presents the isolation, identification and enzymatic screening of heterotrophic bacteria isolated from copper mine drainages. From these samples, 189 bacteria were isolated, which showed diverse catalytic activities. The isolated bacteria were identified by MALDI-TOF and 16S rRNA gene sequencing and several genera were found: Bacillus, Acinetobacter, Pseudomonas, Delftia, Stenotrophomonas, Hydrogenophaga, Rhodococcus, among others. The second chapter presents the catalytic potential of a new epoxide hydrolase from Aspergillus brasiliensis CCT1435 (AbEH), recombinant and expressed in E. coli. This EH is active in a wide pH and temperature range, with a great performance at pH 6.0 and 30 °C. This work also presents the AbEH biocatalytic application for the styrene oxide hydrolysis in aqueous and biphasic media. Finally, the third chapter presents preliminary results involving a chemo-enzymatic method for the production of pyrrolidine alkaloids from the corresponding 1,4-diketones. This approach is promising since several 1,4-diketones were converted to their respective cyclic imines, and the subsequent reduction with NaBH3CN promoted the pyrrolidines formation. Therefore, this thesis presents a broad and diverse study of biocatalytic processes applied in a number of interesting biotechnological reactions (AU)

• FAPESP's process: 11/10378-5 - Purification and functional characterization of copper mine microbial oxidoreductases enzymes
• Grantee: Bruna Zucoloto da Costa
• Support Opportunities: Scholarships in Brazil - Doctorate